Malate Dehydrogenase Activity Assay Kit (Colorimetric) (ab183305)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 0.5 mU/well
製品の概要
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製品名
Malate Dehydrogenase Activity Assay Kit (Colorimetric) -
検出方法
Colorimetric -
サンプルの種類
Tissue, Adherent cells, Suspension cells -
アッセイタイプ
Enzyme activity (quantitative) -
検出感度
< 0.5 mU/well -
種交差性
交差種: Mammals, Other species -
製品の概要
In Abcam's Malate Dehydrogenase Activity Assay kit (Colorimetric) (ab183305), malate dehydrogenase (MDH) reacts with malate to form an intermediate, which in turn reacts with MDH Developer to form a colored product with strong absorbance at 450 nm. The assay is simple, sensitive and can detect less than 0.5 mU of MDH activity in various sample types.
This assay detects both isoforms of MDH: MDH1 and MDH2. If you want to detect enzymatic activity of each specific isoform, please consider MDH1 Activity Assay (ab200009) or MDH2 activity Assay (ab119693).
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K654 Malate Dehydrogenase Activity Colorimetric Assay Kit. K654-100 is the same size as the 100 test size of ab183305.
Malate Dehydrogenase (MDH) (EC 1.1.1.37) is an important enzyme which reversibly converts L-malate into oxaloacetate in the presence of NAD. In eukaryotic cells, malate dehydrogenase has 2 isoforms: MDH1 and MDH2. MDH1 is cytosolic & participates in the malateaspartate shuttle, which transports malate into mitochondria for utilization in ATP generation whereas MDH2 is a mitochondrial enzyme and part of the citric acid cycle. MDH activity is increased in some neurodegenerative diseases such as Alzheimer’s disease, and abnormal MDH activity in serum can serve as a diagnostic tool for severe liver damage (e.g. Hepatocellular carcinoma).
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests MDH Assay Buffer 1 x 25ml MDH Developer (Lyophilized) 1 vial MDH Enzyme Mix (Lyophilized) 1 vial MDH Positive Control (Lyophilized) 1 vial MDH Substrate (Lyophilized) 1 vial NADH Standard (Lyophilized) 1 vial -
研究分野
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細胞内局在
MDH2: Mitochondrion matrix. MDH1: Cytoplasm.
関連製品
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Related Products
画像
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NADH Standard Curve. Malate Dehydrogenase activity in rat muscle extract (0.8 µg), Jurkat cell lysate (0.6 µg), yeast mitochondria (1.2 μg) & MDH Positive Control.
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Rat muscle extract (0.8 μg)
Jurkat cell lysate (0.6 μg)
Yeast mitochondria (1.2 μg)
MDH positive control.
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Referenced MDH Activity in Jurkat cell lysate, rat muscle extract and yeast mitochondrial lysate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab183305 は 3 報の論文で使用されています。
- Chocron ES et al. Mitochondrial TrxR2 regulates metabolism and protects from metabolic disease through enhanced TCA and ETC function. Commun Biol 5:467 (2022). PubMed: 35577894
- Liu X et al. The existence of a nonclassical TCA cycle in the nucleus that wires the metabolic-epigenetic circuitry. Signal Transduct Target Ther 6:375 (2021). PubMed: 34728602
- Teo E et al. Metabolic stress is a primary pathogenic event in transgenic Caenorhabditis elegans expressing pan-neuronal human amyloid beta. Elife 8:N/A (2019). PubMed: 31610847