Anti-MAG/GMA 抗体 [EPR24276-125] (ab277524)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-1-anti-maggma-antibody-epr24276125-immunohistochemistry-human-cerebrum.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24276-125] to MAG/GMA
- Suitable for: WB, IHC-P, IHC-Fr, ICC, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-MAG/GMA antibody [EPR24276-125]
MAG/GMA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR24276-125] to MAG/GMA -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, IHC-Fr, ICC, IPmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Human cerebellum tissue lysate; Mouse brain and cerebellum tissue lysates; Rat cerebellum tissue lysate. IHC-P: Human cerebellum and sciatic nerve tissue; Mouse hippocampus tissue; Rat hippocampus tissue. IHC-Fr: Mouse cerebellum tissue; Rat cerebellum tissue. ICC: Rat primary neural/glia cells. IP: Human cerebellum tissue lysate; Mouse brain tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR24276-125 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab277524の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa).
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| IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Can be used at 1/5000 dilution for human. |
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| IHC-Fr |
1/500.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
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| ICC |
1/50.
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| IP |
1/30.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa). |
|
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Can be used at 1/5000 dilution for human. |
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IHC-Fr
1/500. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
|
ICC
1/50. |
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IP
1/30. |
ターゲット情報
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機能
Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. -
配列類似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4099 Human
- Entrez Gene: 17136 Mouse
- Entrez Gene: 29409 Rat
- Omim: 159460 Human
- SwissProt: P20916 Human
- SwissProt: P20917 Mouse
- SwissProt: P07722 Rat
- Unigene: 643440 Human
see all -
別名
- GMA antibody
- MAG antibody
- MAG_HUMAN antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAG/GMA with ab277524 at 1/5000 dilution (0.1008 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum.The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-8-anti-maggma-antibody-epr24276125-immunohistochemistry-frozen-mouse-cerebellum.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebellum tissue labeling MAG/GMA with ab277524 at 1/500 dilution (1.008 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunocytochemistry - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-5-anti-maggma-antibody-epr24276125-immunocytochemistry-rat-primary-neuralglia-cell.jpg)
Immunocytochemistry - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cell cells labelling MAG/GMA with ab277524 at 1/50 dilution (10.08 ug/ml), followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in rat primary oligodendroglia cells. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Anti-Myelin Basic Protein rat monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
-ve control 1: ab277241 at 1/50 dilution, followed by ab150157 at 1/1000 dilution.
-ve control 2: Anti-Myelin Basic Protein rat monoclonal antibody, followed by ab150080 at 1/1000 dilution.
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![Western blot - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-445433-anti-maggma-antibody-epr24276125-western-blot-lysate.jpg)
Western blot - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)All lanes : Anti-MAG/GMA antibody [EPR24276-125] (ab277524) at 1/1000 dilution
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 69 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
MAG is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 1716323).
Negative control: Liver (PMID: 2432614).
Exposure time: 26 seconds.
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![Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-6-anti-maggma-antibody-epr24276125-immunoprecipitation-human-cerebellum-tissue-lysate10-ug.jpg)
Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)MAG/GMA was immunoprecipitated from 0.35 mg human cerebellum tissue lysate with ab277524 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277524 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human cerebellum tissue lysate 10 ug
Lane 2: ab277524 IP in Human cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab277524 in Human cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 23 seconds.
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![Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-9-anti-maggma-antibody-epr24276125-immunohistochemistry-frozen-rat-cerebellum.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebellum tissue labeling MAG/GMA with ab277524 at 1/500 dilution (1.008 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Western blot - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-445434-anti-maggma-antibody-epr24276125-western-blot-lysate1.jpg)
Western blot - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)All lanes : Anti-MAG/GMA antibody [EPR24276-125] (ab277524) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat cerebellum tissue lysate
Lane 5 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 69 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
MAG is a glycoprotein. The molecular weight observed is consistent with what has been described in the literature (PMID: 1716323).
Negative control: Liver (PMID: 2432614).
Exposure time: 126 seconds.
-
![Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-10-anti-maggma-antibody-epr24276125-immunohistochemistry-frozen-mouse-liver.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling MAG/GMA with ab277524 at 1/100 dilution (5.04 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: No staining on mouse liver (PMID2432614) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-7-anti-maggma-antibody-epr24276125-immunoprecipitation-mouse-brain-tissue-lysate10-ug.jpg)
Immunoprecipitation - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)MAG/GMA was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab277524 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab277524 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: ab277524 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab277524 in Mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 23 seconds.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-2-anti-maggma-antibody-epr24276125-immunohistochemistry-hu-sciatic-nerve.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)Immunohistochemical analysis of paraffin-embedded human sciatic nerve tissue labeling MAG/GMA with ab277524 at 1/5000 dilution (0.1008 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human sciatic nerve. The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-11-anti-maggma-antibody-epr24276125-immunohistochemistry-frozen-rat-liver.jpg)
Immunohistochemistry (Frozen sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver tissue labeling MAG/GMA with ab277524 at 1/100 dilution (5.04 ug/ml) followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: No staining on rat liver (PMID2432614) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-3-anti-maggma-antibody-epr24276125-immunohistochemistry-mouse-hippocampus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling MAG/GMA with ab277524 at 1/2000 dilution (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hippocampus. (PMID: 25523827)The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-4-anti-maggma-antibody-epr24276125-immunohistochemistry-rat-hippocampus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAG/GMA antibody [EPR24276-125] (ab277524)Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling MAG/GMA with ab277524 at 1/2000 (0.252 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hippocampus. (PMID: 17705198, 27000654 )The section was incubated with ab277524 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Anti-MAG/GMA antibody [EPR24276-125] (ab277524)](https://www.abcam.co.jp/ps/products/277/ab277524/Images/ab277524-447401-benefitsofrecombinantantibodies.png)
Anti-MAG/GMA antibody [EPR24276-125] (ab277524)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab277524 は論文での使用が確認できていません。
