Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na Citrate pH 6.0
Permeabilization
No
Specification
pancreas
Fixative
10% NBF
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Leica BOND primary dilutent
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Leica BOND HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Attempts to replicate online review results failed. We saw no staining of B-cell nuclei on mouse FFPE pancreas sections with tissues stained for 15 or 30 minutes @ RT with a 1:1000 dilution of ab17976. We do, however, see staining after overnight incubation at room temperature. Unfortunately, the staining is cytoplasmic, not nuclear as shown in prior reviews. This was constant with 1:100 to 1:1000 dilutions as well as pH 9.0 HIER treatment. Staining this tissue with ab85008 shows expected, nuclear, localization of MafA protein. We would expect wild-type normoglycemic animals to have nuclear MafA localization.
Abcam response
We would like to thank this researcher for submitting their review. ab17976 is guaranteed for IHC-P with mouse samples. This researcher received ab85008 as free of charge replacement under our Abpromise guarantee.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. David Burk
Verified customer
投稿 Nov 26 2018