Anti-MafA 抗体 (ab17976)
Key features and details
- Rabbit polyclonal to MafA
- Suitable for: WB, EMSA
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MafA antibody
MafA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MafA -
由来種
Rabbit -
アプリケーション
適用あり: WB, EMSAmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
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免疫原
Synthetic peptide corresponding to Mouse MafA (C terminal).
Database link: Q8CF90 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17976の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000.
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| EMSA |
Use a concentration of 0.05 - 0.25 mg/ml.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. |
|
EMSA
Use a concentration of 0.05 - 0.25 mg/ml. |
ターゲット情報
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機能
Acts as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context. -
配列類似性
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain. -
翻訳後修飾
Ubiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway. -
細胞内局在
Nucleus. Detected in nuclei of pancreas islet beta cells. - Information by UniProt
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参照データベース
- Entrez Gene: 389692 Human
- Entrez Gene: 366949 Rat
- Omim: 610303 Human
- SwissProt: Q8NHW3 Human
- Unigene: 521914 Human
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別名
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
see all
画像
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Western blot - Anti-MafA antibody (ab17976)
Detection of MafA, MafB and cMaf by Western Blot. Samples: Nuclear extract (6 µg) from HeLa cells transfected with MafA, MafB or cMaf expression constructs. Antibodies: Affinity purified anti-MafA antibody, anti-MafB Antibody or anti-c-Maf antibody. Each antibody was used at 0.5 µg/ml. Detection: Chemiluminescence with a 5 second exposure.
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Electrophoretic Mobility Shift Assay - Anti-MafA antibody (ab17976)Electrophoretic Mobility Shift of MafA, MafB and c-Maf. Samples: Nuclear extract (6 ug) from HeLa cells transfected with MafA, MafB or c-Maf expression constructs. Antibodies: Affinity purified anti-MafA antibody BL1069, anti-MafB Antibody or anti-c-Maf antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab17976 は 5 報の論文で使用されています。
- Xu J et al. MAFB mediates the therapeutic effect of sleeve gastrectomy for obese diabetes mellitus by activation of FXR expression. Braz J Med Biol Res 51:e7312 (2018). PubMed: 29846411
- Roth Flach RJ et al. Protein Kinase Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Promotes Obesity-induced Hyperinsulinemia. J Biol Chem 291:16221-30 (2016). IHC-P ; Mouse . PubMed: 27226575
- Sakano D et al. VMAT2 identified as a regulator of late-stage ß-cell differentiation. Nat Chem Biol 10:141-8 (2014). PubMed: 24316738
- Hunter KE et al. Identification and characterization of poorly differentiated invasive carcinomas in a mouse model of pancreatic neuroendocrine tumorigenesis. PLoS One 8:e64472 (2013). WB, IHC-P ; Mouse . PubMed: 23691228
- Vargas N et al. TAT-Mediated Transduction of MafA Protein In Utero Results in Enhanced Pancreatic Insulin Expression and Changes in Islet Morphology. PLoS One 6:e22364 (2011). WB ; Mouse . PubMed: 21857924