Anti-Mael 抗体 (ab106713)
Key features and details
- Rabbit polyclonal to Mael
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Mael antibody -
製品の詳細
Rabbit polyclonal to Mael -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
A 16 amino acid peptide near the C terminus of Human Mael (NP_065199).
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ポジティブ･コントロール
- Human skeletal muscle tissue; HeLa cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at 4°C (stable for up to 12 months). -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106713の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa.
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| ICC |
Use a concentration of 5 µg/ml.
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| ICC/IF |
Use a concentration of 20 µg/ml.
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| 特記事項 |
|---|
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa. |
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ICC
Use a concentration of 5 µg/ml. |
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ICC/IF
Use a concentration of 20 µg/ml. |
ターゲット情報
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機能
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for localization of germ-cell factors to the meiotic nuage. -
組織特異性
Testis-specific. Expressed in various cancer cell lines, probably due to demethylation of its promoter. -
配列類似性
Belongs to the maelstrom family.
Contains 1 HMG box DNA-binding domain. -
細胞内局在
Cytoplasm. Nucleus. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs. - Information by UniProt
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参照データベース
- Entrez Gene: 84944 Human
- Omim: 611368 Human
- SwissProt: Q96JY0 Human
- Unigene: 651245 Human
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別名
- cancer/testis antigen 128 antibody
- CT128 antibody
- FLJ14904 antibody
see all
画像
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Western blot - Anti-Mael antibody (ab106713)Anti-Mael antibody (ab106713) at 1 µg/ml + HeLa cell lysate at 15 µg
Predicted band size: 49 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Mael antibody (ab106713)Immunofluorescence of Mael in HeLa cells with ab106713 at 20 ug/mL.
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Immunocytochemistry - Anti-Mael antibody (ab106713)ab106713, at 5 µg/ml, staining in Hela cells with Maelstrom by Immunocytochemistry.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab106713 は 3 報の論文で使用されています。
- Rocha-da-Silva L et al. Expression of genome defence protein members in proliferating and quiescent rat male germ cells and the Nuage dynamics. PLoS One 14:e0217941 (2019). PubMed: 31181099
- Bothun AM et al. Quantitative Proteomic Profiling of the Human Ovary from Early to Mid-Gestation Reveals Protein Expression Dynamics of Oogenesis and Folliculogenesis. Stem Cells Dev 27:723-735 (2018). PubMed: 29631484
- Cao J et al. High expression of piwi-like RNA-mediated gene silencing 1 is associated with poor prognosis via regulating transforming growth factor-ß receptors and cyclin-dependent kinases in breast cancer. Mol Med Rep 13:2829-35 (2016). PubMed: 26847393
