Anti-MAdCAM1 抗体 [AP-MAB0842] (ab80680)
Key features and details
- Rat monoclonal [AP-MAB0842] to MAdCAM1
- Suitable for: IHC-Fr, IP, IHC-P, Flow Cyt, Inhibition Assay
- Reacts with: Mouse
- Isotype: IgG2
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MAdCAM1 antibody [AP-MAB0842]
MAdCAM1 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [AP-MAB0842] to MAdCAM1 -
由来種
Rat -
アプリケーション
適用あり: IHC-Fr, IP, IHC-P, Flow Cyt, Inhibition Assaymore details -
種交差性
交差種: Mouse -
免疫原
Mouse Endothelial cells
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特記事項
Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
The IgG fraction of culture supernatant was purified by Protein A affinity chromatography. -
一次抗体 備考
ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases. -
ポリ/モノ
モノクローナル -
クローン名
AP-MAB0842 -
アイソタイプ
IgG2 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80680の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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|
IHC-P | (1) |
Use at an assay dependent concentration.
|
Flow Cyt |
Use at an assay dependent concentration.
|
|
Inhibition Assay |
Use at an assay dependent concentration.
ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor. |
特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Inhibition Assay
Use at an assay dependent concentration. ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor. |
ターゲット情報
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機能
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. -
組織特異性
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. -
配列類似性
Contains 2 Ig-like (immunoglobulin-like) domains. -
翻訳後修飾
The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 17123 Mouse
- SwissProt: Q61826 Mouse
- Unigene: 391556 Mouse
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別名
- Addressin mucosal antibody
- hMAdCAM 1 antibody
- hMAdCAM-1 antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (1)
ab80680 は 1 報の論文で使用されています。
- Gao RY et al. Bile acids modulate colonic MAdCAM-1 expression in a murine model of combined cholestasis and colitis. Mucosal Immunol 14:479-490 (2021). PubMed: 33004979