Anti-Mad2L2/REV7 抗体 (ab3630)
Key features and details
- Rabbit polyclonal to Mad2L2/REV7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Mad2L2/REV7 antibody
Mad2L2/REV7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Mad2L2/REV7 -
由来種
Rabbit -
特異性
This antibody detects a single band just below the 21kD marker, that corresponds to Mad2L2/REV7.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Xenopus laevis
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免疫原
Synthetic peptide corresponding to Human Mad2L2/REV7 aa 3-14.
Sequence:TLTRQDLNFGQV
(Peptide available asab11254) -
特記事項
This product was previously labelled as Mad2L2
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab3630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Mad2L2/REV7 peptide (ab11254). |
ターゲット情報
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機能
Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. -
組織特異性
Ubiquitously expressed. -
配列類似性
Contains 1 HORMA domain. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 10459 Human
- Entrez Gene: 394380 Xenopus laevis
- Omim: 604094 Human
- SwissProt: Q9UI95 Human
- SwissProt: Q8QFR4 Xenopus laevis
- Unigene: 19400 Human
- Unigene: 8200 Xenopus laevis
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別名
- Homolog of REV7 S cerevisiae antibody
- hREV7 antibody
- MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
see all
画像
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Western blot - Anti-Mad2L2/REV7 antibody (ab3630)All lanes : Anti-Mad2L2/REV7 antibody (ab3630) at 1/500 dilution
Lane 1 : Whole cell extract prepared from Hela cells
Lane 2 : Nuclear lysates prepared from Hela cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 24 kDaWestern blot with ab3630 at 1/500.
Secondary: ab6721 at 1/2000.
Exposure: 30 sec.
Lysates at 20 µg/lane.Lane 1: HeLa Whole Cell extract.
Lane 2: HeLa Nuclear extract.
データシートおよび資料
参考文献 (0)
ab3630 は論文での使用が確認できていません。
