Anti-Mad2L2/REV7 抗体 (ab3630)

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Western blot - Anti-Mad2L2/REV7 antibody (ab3630)

    Key features and details

    • Rabbit polyclonal to Mad2L2/REV7
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Mad2L2/REV7 antibody
      Mad2L2/REV7 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Mad2L2/REV7

    • 由来種

      Rabbit

    • 特異性

      This antibody detects a single band just below the 21kD marker, that corresponds to Mad2L2/REV7.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Xenopus laevis

    • 免疫原

      Synthetic peptide corresponding to Human Mad2L2/REV7 aa 3-14.
      Sequence:

      TLTRQDLNFGQV


      (Peptide available as ab11254)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

       This product was previously labelled as Mad2L2

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3630の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/500. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Mad2L2/REV7 peptide (ab11254).
    特記事項
    WB
    1/500. Detects a band of approximately 20 kDa (predicted molecular weight: 24 kDa).Can be blocked with Human Mad2L2/REV7 peptide (ab11254).

    ターゲット情報

    • 機能

      Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.

    • 組織特異性

      Ubiquitously expressed.

    • 配列類似性

      Contains 1 HORMA domain.

    • 細胞内局在

      Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm.
    • Information by UniProt

    • 参照データベース

      • 別名

        • Homolog of REV7 S cerevisiae antibody
        • hREV7 antibody
        • MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
        • MAD2 homolog antibody
        • MAD2 like 2 antibody
        • MAD2 mitotic arrest deficient like 2 antibody
        • MAD2-like protein 2 antibody
        • MAD2B antibody
        • Mad2l2 antibody
        • MD2L2_HUMAN antibody
        • Mitotic Arrest Deficient 2 L2 antibody
        • Mitotic arrest deficient 2-like protein 2 antibody
        • Mitotic arrest deficient homolog like 2 antibody
        • Mitotic arrest deficient like 2 (yeast) antibody
        • Mitotic arrest deficient yeast homolog antibody
        • Mitotic spindle assembly checkpoint protein MAD2B antibody
        • Polymerase (DNA directed) zeta 2 accessory subunit antibody
        • POLZ2 antibody
        • REV 7 antibody
        • REV7 antibody
        • REV7 homolog antibody
        • Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody
        see all

      画像

      • Western blot - Anti-Mad2L2/REV7 antibody (ab3630)
        Western blot - Anti-Mad2L2/REV7 antibody (ab3630)
        All lanes : Anti-Mad2L2/REV7 antibody (ab3630) at 1/500 dilution

        Lane 1 : Whole cell extract prepared from Hela cells
        Lane 2 : Nuclear lysates prepared from Hela cells

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

        Predicted band size: 24 kDa



        Western blot with ab3630 at 1/500.

        Secondary: ab6721 at 1/2000.
        Exposure: 30 sec.
        Lysates at 20 µg/lane.

        Lane 1: HeLa Whole Cell extract.
        Lane 2: HeLa Nuclear extract.

      参考文献 (0)

      ab3630 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3630 は論文での使用が確認できていません。

      レビューと Q&A

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      レビューを送る 質問を送る

      Western blot abreview for Anti-Mad2L2/REV7 antibody

       Inconclusive
      Abreviews
      Application
      Western blot
      Sample
      Zebrafish Tissue lysate - whole (whole larvae)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      100 µg
      Specification
      whole larvae
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Mr. Gregory Weber

      Verified customer

      投稿 Aug 04 2011

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