Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Mouse Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
This antibody does not appear to recognize any kinetochore associated protein as no localization was observed in mitotic cells (prophase to metaphase).
Abcam response
Thank you for your valuable feedback. ICC/IF was not a tested application for ab10691 and it seems that this antibody does not recognize Mad2L1 under these experimental conditions. Thank you for your time, and we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Dec 18 2006