Anti-MAD1L1/MAD1 抗体 [9B10] (ab5783)
![Flow Cytometry - Anti-MAD1L1/MAD1 antibody [9B10] (ab5783)](https://www.abcam.co.jp/ps/products/5/ab5783/Images/ab5783-195393-anti-mad1-antibody-9b10-flow-cytometry.jpg "Flow Cytometry - Anti-MAD1L1/MAD1 antibody [9B10] (ab5783)")
Key features and details
- Mouse monoclonal [9B10] to MAD1L1/MAD1
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-MAD1L1/MAD1 antibody [9B10]
MAD1L1/MAD1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [9B10] to MAD1L1/MAD1 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cytmore details
適用なし: WB -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human MAD1L1/MAD1.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
9B10 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5783の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use 0.1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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| 特記事項 |
|---|
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Flow Cyt
Use 0.1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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追加情報
Is unsuitable for WB.
ターゲット情報
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機能
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. -
組織特異性
Expressed weakly at G0/G1 and highly at late S and G2/M phase. -
関連疾患
Defects in MAD1L1 are involved in the development and/or progression of various types of cancer. -
配列類似性
Belongs to the MAD1 family. -
翻訳後修飾
Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. -
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore. - Information by UniProt
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参照データベース
- Entrez Gene: 8379 Human
- Omim: 602686 Human
- SwissProt: Q9Y6D9 Human
- Unigene: 654838 Human
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別名
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
see all
画像
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Flow Cytometry - Anti-MAD1L1/MAD1 antibody [9B10] (ab5783)Overlay histogram showing HeLa cells stained with ab5783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5783, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab5783 は 5 報の論文で使用されています。
- Petsalaki E et al. The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling. J Cell Biol 217:861-876 (2018). PubMed: 29362225
- Monda JK & Cheeseman IM Dynamic regulation of dynein localization revealed by small molecule inhibitors of ubiquitination enzymes. Open Biol 8:N/A (2018). PubMed: 30257893
- Vanarsdall AL et al. CD147 Promotes Entry of Pentamer-Expressing Human Cytomegalovirus into Epithelial and Endothelial Cells. mBio 9:N/A (2018). PubMed: 29739904
- Rajanala K et al. Phosphorylation of nucleoporin Tpr governs its differential localization and is required for its mitotic function. J Cell Sci 127:3505-20 (2014). PubMed: 24938596
- Hewitt L et al. Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex. J Cell Biol 190:25-34 (2010). ICC/IF ; Human . PubMed: 20624899