Anti-MACF1 抗体 (ab117418)
Key features and details
- Rabbit polyclonal to MACF1
- Suitable for: IHC-P, ICC/IF, WB, IP, ELISA
- Reacts with: Mouse
- Isotype: IgG
製品の概要
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製品名
Anti-MACF1 antibody
MACF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MACF1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WB, IP, ELISAmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat -
免疫原
Synthetic peptide corresponding to Mouse MACF1 aa 3650-3850.
Database link: Q9QXZ0 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Constituent: Proprietary component -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab117418の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/250.
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|
ICC/IF |
1/250.
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|
WB |
1/500. Predicted molecular weight: 838 kDa.
|
|
IP |
1/200.
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|
ELISA |
1/10000.
|
特記事項 |
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IHC-P
1/250. |
ICC/IF
1/250. |
WB
1/500. Predicted molecular weight: 838 kDa. |
IP
1/200. |
ELISA
1/10000. |
ターゲット情報
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関連性
Microtubule-actin crosslinking factor 1 (MACF1/ACF7) belongs to spectraplakin family of proteins which also includes mammalian Bpag1/dystonin, Drosophila Short Stop (Shot) and Caenorhabditis elegans Vab-10. These are large actin-microtubule linker molecules that are essential for various biological processes such as neuronal degeneration, wound healing, gastrulation. MACF1, located on mouse chromosome 4D2.2, was found to play an important role during neurogenesis- it was shown that MACF1 regulates the organization of neuronal microtubules which is essential for axon extension. Furthermore, MACF1 is required in controlling focal adhesion assembly and dynamics. MACF1 behaves as an actin-regulated ATPase and it has the ability to target microtubules that track along F-actin to focal adhesions. MACF1 was also implicated in regulating filopodia formation. -
細胞内局在
Cytoplasmic, microtubule. Plasma membrane. Golgi apparatus. -
参照データベース
- Entrez Gene: 11426 Mouse
- Entrez Gene: 362587 Rat
- SwissProt: Q9QXZ0 Mouse
- SwissProt: D3ZHV2 Rat
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別名
- ABP620 antibody
- Acf7 antibody
- Aclp7 antibody
see all
画像
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (7)
ab117418 は 7 報の論文で使用されています。
- Li D et al. Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging. Theranostics 11:5585-5604 (2021). PubMed: 33859765
- Yin C et al. MACF1 alleviates aging-related osteoporosis via HES1. J Cell Mol Med N/A:N/A (2021). PubMed: 34133068
- Zhao F et al. Mesenchymal MACF1 Facilitates SMAD7 Nuclear Translocation to Drive Bone Formation. Cells 9:N/A (2020). PubMed: 32143362
- Lin X et al. Microtubule actin crosslinking factor 1 (MACF1) knockdown inhibits RANKL-induced osteoclastogenesis via Akt/GSK3ß/NFATc1 signalling pathway. Mol Cell Endocrinol 494:110494 (2019). PubMed: 31260729
- Oury J et al. MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses. J Cell Biol 218:1686-1705 (2019). PubMed: 30842214
- Yin C et al. Mechanical unloading reduces microtubule actin crosslinking factor 1 expression to inhibit β-catenin signaling and osteoblast proliferation. J Cell Physiol 233:5405-5419 (2018). PubMed: 29219183
- Xie SD et al. [MACF1 knockdown in glioblastoma multiforme cells increases temozolomide-induced cytotoxicity]. Nan Fang Yi Ke Da Xue Xue Bao 37:1183-1189 (2017). PubMed: 28951359