Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549)
Specification
A549
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton
Blocking step
5% Goat Serum, 0.5% BSA, 0.5% Gelatin as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/350
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Secondary antibody
Name
Non-Abcam antibody was used: Cy5- conjugated AffiniPure Goat anti-mouse IgG1 Sp
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ®
Dilution
1/400
Additional data
Additional Notes
The nucleus was stained with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Jul 07 2010