Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK 293T cells)
Permeabilization
Yes - 0.1% Saponin, 20% FCS in PBS
Specification
HEK 293T cells
Blocking step
0.1% Saponin, 20% FCS in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 21°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 0.1% Saponin, 20% FCS in PBS
Dilution
1/200
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Alexa Fluor 488 Donkey anti-Rabbit Invitrogen A212
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Monoclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Excellent antibody for immunofluorescent labelling of CIMPR. I have tested this antibody in HeLa, HEK 293T and U2OS cells with great success.
The previous LOT of this antibody has stored nicely at -20C throughout its use over the course of a year without any noticeable loss of specificity. The current LOT has performed just as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 18 2019