Application
Western blot
Sample
Hamster Cell lysate - other (BHK cell post-nuclear supernatant)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
200000 cells
Specification
BHK cell post-nuclear supernatant
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
4 µg/ml
Detection
Detection method
ECL
Exposure
24 second(s)
Positive control
Blot of same sample with ab32815 yielded band at 250-275 kDA.
Additional data
Additional Notes
Hamster cell post-nuclear supernatant does not stain with ab12894 but does stain with ab32815
Abcam response
Abcam has not tested ab12894 in Syrian Hamster samples and thus can't say how it should have performed. This individual received a credit for testing this antibody in Syrian Hamster samples as part of our AbTrial program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 08 2019