Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.1% Triton X-100
Specification
HeLa
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% BSA in PBS with 0.1% Triton X-100
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Invitrogen Rabbit anti Mouse Alexa Fluor 594
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
HeLa cells were fixed with 4% PFA for 15 minutes, followed by blocking/permeabilization in PBS with 5% Normal Goat Serum/0.1% Triton X-100.
Samples were analyzed by spinning disk confocal microscopy. Image shown represents a maximum intensity projection of a 2 micron z-stack with a step size of 0.2 um. M6PR signal (shown in magenta) is punctate/vesicular. The Golgi marker alpha-Mannosidase is shown in green, and Nuclei are stained with DAPI (blue).
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投稿 Oct 11 2018