アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報

新型コロナウイルス (COVID-19)に伴う当社の対応について

  1. Link

    m13--fd-bacteriophage-coat-proteins-antibody-ab6188.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Organism Virus DNA Virus single stranded DNA Virus Enterobacteria phage M13
Share by email

Anti-M13 + Fd Bacteriophage Coat Proteins 抗体 (ab6188)

  • Datasheet
  • SDS
Reviews (2)Q&A (9)References (9)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Rabbit polyclonal to M13 + Fd Bacteriophage Coat Proteins
  • Suitable for: Indirect ELISA, ELISA
  • Isotype: IgG

こちらの製品もご検討ください

標識
Product image
Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
タンパク質
Product image
Streptavidin (HRP) (ab7403)

関連製品

製品の概要

  • 製品名

    Anti-M13 + Fd Bacteriophage Coat Proteins antibody
  • 製品の詳細

    Rabbit polyclonal to M13 + Fd Bacteriophage Coat Proteins
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: Indirect ELISA, ELISAmore details
  • 種交差性

    交差種: Other species
  • 免疫原

    fd bacteriophage

  • 特記事項

    Concentration: 5.5 mg/ml- 7.5 mg/ml


    Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • 精製度

    IgG fraction
  • 一次抗体 備考

    Can be used as a capture antibody when coated directly on microtiter plates, or as a primary detection antibody for specifically captured M13 or fd phage. May be useful in rapidly sorting large phage display libraries (antibody, peptide etc.) with the expressed proteins fused to either the gene III or gene VIII protein of the filamentous phage. May be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • single stranded DNA Virus
    • Enterobacteria phage M13

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

Our Abpromise guarantee covers the use of ab6188 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Indirect ELISA Use at an assay dependent concentration. At least 1/1000 for 5 x107 phage/mlat least 1/8000 for 5 x 1010 phage/ml.
ELISA Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends.
  • 参照データベース

    • Entrez Gene: 927331 Other species
    • Entrez Gene: 927332 Other species
    • Entrez Gene: 927333 Other species
    • Entrez Gene: 927334 Other species
    • Entrez Gene: 927335 Other species
    • GenBank: J02451.1 Other species
    • SwissProt: P69168 Other species
    • SwissProt: P69532 Other species
    • SwissProt: P69535 Other species
    • SwissProt: P69538 Other species
    • SwissProt: P69541 Other species
    see all
  • 別名

    • Coat protein A antibody
    • Coat protein B antibody
    • Coat protein C polypeptide I antibody
    • Coat protein C polypeptide II antibody
    • Coat protein D antibody
    • M13 coat protein A antibody
    • M13 coat protein B antibody
    • M13 coat protein C polypeptide I antibody
    • M13 coat protein C polypeptide II antibody
    • M13 coat protein D antibody
    • M13 minor coat protein antibody
    • Minor coat protein antibody
    see all

プロトコール

  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (9)

    ab6188 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6188 は 9 報の論文で使用されています。

    • Li X  et al. A novel peptide targets CD105 for tumour imaging in vivo. Oncol Rep 40:2935-2943 (2018). PubMed: 30132552
    • Phipps ML  et al. Beyond Helper Phage: Using "Helper Cells" to Select Peptide Affinity Ligands. PLoS One 11:e0160940 (2016). PubMed: 27626637
    • Vargas-Sanchez K  et al. DNA Subtraction of In Vivo Selected Phage Repertoires for Efficient Peptide Pathology Biomarker Identification in Neuroinflammation Multiple Sclerosis Model. Biomark Insights 11:19-29 (2016). PubMed: 26917946
    • Costantini TW  et al. Intravenous phage display identifies peptide sequences that target the burn-injured intestine. Peptides 38:94-9 (2012). PubMed: 22960048
    • Wu D  et al. Phage Displayed Peptides to Avian H5N1 Virus Distinguished the Virus from Other Viruses. PLoS One 6:e23058 (2011). ELISA . PubMed: 21887228
    • Antonara S  et al. Borrelia burgdorferi adhesins identified using in vivo phage display. Mol Microbiol 66:262-76 (2007). ELISA . PubMed: 17784908
    • Kelly KA  et al. SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration. J Leukoc Biol 81:748-56 (2007). ICC/IF . PubMed: 17178915
    • Hekim C  et al. Novel peptide inhibitors of human kallikrein 2. J Biol Chem 281:12555-60 (2006). PubMed: 16527822
    • Kelly KA  et al. Detection of vascular adhesion molecule-1 expression using a novel multimodal nanoparticle. Circ Res 96:327-36 (2005). PubMed: 15653572

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 11 Abreviews or Q&A

    ELISA abreview for Anti-M13 + Fd Bacteriophage Coat Proteins antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Bacteria Recombinant protein (filamentous bacteriophage from host E. coli strain)
    Specification
    filamentous bacteriophage from host E. coli strain
    Blocking step
    BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Indirect
    Read More

    Mr. Chang Su Jeon

    Verified customer

    投稿 Jul 27 2015

    Question

    I would like to get more vials of lot 861626. How many more are available?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 16 2011

    Answer

    Unfortunately lot 861626 is an individual batch which was not produced from a parent batch, also I'm afraid that we no longer have stock of this batch in Europe or the USA. Sorry that I don't have better news; if you have any further questions please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Dec 16 2011

    Question

     Hi, I am wondering if peptide mimotope of your Anti-M13 + Fd Bacteriophage Coat Proteins antibody is known?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 31 2011

    Answer

    Thank you very much for your inquiry. The immunogen for this polyclonal antibody was a cell preparation of fd bacteriophage. We therefore do not know what epitope is bound exactly by this antibody. Therefore we also cannot make any predictions about the possible mimotopes (A mimotope is a macromolecule which mimics the structure of an epitope. Because of this property it causes an antibody response identical to the one elicited by the epitope.) that are recognized by this antibody. I am sorry I could not be of more help on this occasion and hope this information was nevertheless helpful. Please do not hesitate to contact me again with any further questions.

    Read More

    Abcam Scientific Support

    Answered on Oct 31 2011

    Question

    Thank you very much for reply. So do you supply the anti-pVIII protein of M13 phage antibody for ELISA (used in phage display experiment)? And HRP/AntiM13 and HRP substrate OPD (O-phenyldiamine dihydrochloride)?   Thank you very much.  

    Read More

    Abcam community

    Verified customer

    Asked on Sep 15 2011

    Answer

    Thank you for contacting us. We have two anti-M13 antibodies that may be of interest: ab6188 Anti-M13 + Fd Bacteriophage Coat Proteins antibody Binds specifically to phage coat proteins of M13 or fd phage. Click here (or use the following: https://www.abcam.com/M13--Fd-Bacteriophage-Coat-Proteins-antibody-ab6188.html). ab50370 Anti-M13 antibody [B62-FE2] (HRP) Binds to an epitope on pVIII (phage coat protein) covering the N-Terminal region of the g8p AEGDDPAKAAFDSLQASAT. Click here (or use the following: https://www.abcam.com/HRP-M13-antibody-B62-FE2-ab50370.html). We have a number of HRP kits, mainly for IHC. Here is a link to a HRP substrate than can be used for WB: ab5801 Chemiluminescent HRP Substrate (membrane) Click here (or use the following: https://www.abcam.com/Chemiluminescent-HRP-Substrate-membrane-ab5801.html). Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Sep 15 2011

    Question

    Do you have antibodies for phage proteins?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 14 2011

    Answer

    Thank you for your reply. We have a number of antibodies to Bacteriophage Proteins such as ab6188: Anti-M13 + Fd Bacteriophage Coat Proteins antibody or ab9225: Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2]. I hope this helps, please let me know if you need any additional information.

    Read More

    Abcam Scientific Support

    Answered on Sep 14 2011

    ELISA abreview for Anti-M13 + Fd Bacteriophage Coat Proteins antibody

    Excellent
    Abreviews
    Abreviews
    Application
    ELISA
    Sample
    Rabbit Recombinant protein (phage coat proteins of M13)
    Specification
    phage coat proteins of M13
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 27°C
    Type
    Sandwich (Capture)
    Read More

    Miss. I-Hsuan Chen

    Verified customer

    投稿 Jul 09 2010

    Question

    I have a question regarding antibody ab6188 (M13 bacteriophage coat proteins). We used this antibody in immunofluorescence stainings and observed an intense cross-reactivity with (non M13-infected) E. coli, apparently with one of the outer membrane proteins. In fact, it was the major signal (see attached pictures). We used the antibody in a 1:50 dilution in PBS and used skim milk powder as blocking reagent. Detection was with an Alexa Fluor 488 - coupled secondary antibody. A negative control (ab6188 omitted) resulted in zero fluorescence, as expected. As we want to use the antibody in part to discriminate between infected and non-infected E. coli, this is clearly unacceptable. Do you have any suggestion how to solve this problem?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 16 2006

    Answer

    Thank you for your enquiry. I have been in correspondence with the source of this antibody. Following discussion we concluded that there may be a degree of contamination in your samples. There is clear specific reactivity with your M13- cells. Can you tell me whether it is a possibility that this may have happened? This is supported by the fact that there is variable fluorescence in the cells, possibly reflective of the variable copy number in the M13 infection. I consider it unlikely that this antibody is demonstrating reactivity with non-specific E.coli proteins. I appreciate your comments.

    Read More

    Abcam Scientific Support

    Answered on Nov 17 2006

    Question

    I have your ab6188 antibody. Was wondering if aliquoting with ethylene glycol will improve its life?

    Read More

    Abcam community

    Verified customer

    Asked on Nov 01 2005

    Answer

    Thank you for your enquiry. We have not tested the stability of the antibody ab6188 when stored in other buffer than its storage buffer so we do not know if it will improve or damage its storage conditions. In my experience it is best to use glycerol (final volume 50%) if you really wish to dilute and protect it. I hope this information helps you,

    Read More

    Abcam Scientific Support

    Answered on Nov 02 2005

    Question

    I have technical difficult with the other anti-phage M13 antibodies and I was wondering if I can have a samples of your antibody to test it for immunofluorescence staining in frozen tissues if this has not been done before.

    Read More

    Abcam community

    Verified customer

    Asked on Mar 28 2005

    Answer

    Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to test an antibody in an application for which we do not have any information, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards.

    Read More

    Abcam Scientific Support

    Answered on Mar 28 2005

    Question

    Can you please tell me the concentration of your 'M13 Bacteriophage Coat Proteins' antibody #AB6188? It is an IgG fraction, so I hope it has been measured. Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 11 2004

    Answer

    I apologise for taking so long I have been awaiting confirmation of the concentration. The concentration of the product is batch dependent and will range from 5.5 mg/ml to 7.5 mg/ml. I hope that this information is useful to you. If I can be of any further assistance please let me know.

    Read More

    Abcam Scientific Support

    Answered on Aug 19 2004

    1-10 of 11 Abreviews or Q&A

    •  戻る
    • 1
    • 2
    • 次へ 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.