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Anti-M-CSF 抗体 (ab9693)

Reviews (1)Q&A (1)References (19)
Western blot - Anti-M-CSF antibody (ab9693)
  • Western blot - Anti-M-CSF antibody (ab9693)
  • Sandwich ELISA - Anti-M-CSF antibody (ab9693)

Key features and details

  • Rabbit polyclonal to M-CSF
  • Suitable for: WB, IHC-P, ELISA, Neutralising, Sandwich ELISA
  • Reacts with: Human, Recombinant fragment
  • Isotype: unknown

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-M-CSF antibody [EP1179Y] (ab52864)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-M-CSF antibody
    M-CSF 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to M-CSF

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ELISA, Neutralising, Sandwich ELISAmore details

  • 種交差性

    交差種: Human, Recombinant fragment

  • 免疫原

    Recombinant full length protein corresponding to Human M-CSF. Highly pure (>98%) recombinant hM-CSF (human M-CSF)
    Database link: P09603

  • ポジティブ･コントロール

  • 特記事項

    This product is no longer batch tested in IHC, for an IHC validated antibody please see ab52864

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9693の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
Use at an assay dependent concentration. To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

To detect hM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF.
Neutralising
Use at an assay dependent concentration.

To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 µg/ml of this antibody is required.
Sandwich ELISA
Use a concentration of 0.1 µg/ml.

To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.
特記事項
WB
Use at an assay dependent concentration. To detect hM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hM-CSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
IHC-P
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

To detect hM-CSF by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hM-CSF.
Neutralising
Use at an assay dependent concentration.

To yield one-half maximal inhibition [ND50] of the biological activity of hM-CSF (7.0 ng/ml), a concentration of 0.02 - 0.03 µg/ml of this antibody is required.
Sandwich ELISA
Use a concentration of 0.1 µg/ml.

To detect hM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required.

ターゲット情報

  • 機能

    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.

  • 翻訳後修飾

    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.

  • 細胞内局在

    Cell membrane and Secreted > extracellular space.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Colony stimulating factor 1 (macrophage) antibody
      • Colony stimulating factor 1 antibody
      • Colony stimulating factor macrophage specific antibody
      • CSF 1 antibody
      • CSF-1 antibody
      • CSF1 antibody
      • CSF1_HUMAN antibody
      • Csfm antibody
      • Lanimostim antibody
      • M CSF antibody
      • M-CSF antibody
      • Macrophage Colony Stimulating Factor 1 antibody
      • Macrophage colony stimulating factor antibody
      • MCSF antibody
      • MGC31930 antibody
      • Processed macrophage colony-stimulating factor 1 antibody
      see all

    画像

    • Western blot - Anti-M-CSF antibody (ab9693)
      Western blot - Anti-M-CSF antibody (ab9693)
    • Western blot - Anti-M-CSF antibody (ab9693)
      Western blot - Anti-M-CSF antibody (ab9693)This image is courtesy of an Abreview submitted by Valentina Marcelli
      All lanes : Anti-M-CSF antibody (ab9693) at 1/500 dilution (for 12 hours at 4°C)

      Lane 1 : Human Kidney whole cell lysate
      Lane 2 : Human Kidney whole cell lysate transfected with LFABP

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : An IRDye®800CW-conjugated Goat anti-rabbit IgG polyclonal at 1/15000 dilution

      Performed under non-reducing conditions.

      Observed band size: 70 kDa why is the actual band size different from the predicted?



      Blocking Step: 5% BSA for 1 hour at 25°C

      Deatection method: Odyssey for 50 seconds.

      Right-hand Image: Weaker exposure to identify bands.

      See Abreview

    • Sandwich ELISA - Anti-M-CSF antibody (ab9693)
      Sandwich ELISA - Anti-M-CSF antibody (ab9693)

      Standard curve for MCSF (Analyte: ab62015); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [21] to MCSF (ab18645) at 1µg/ml and Detector Antibody Rabbit polyclonal to MCSF (ab9693) at 0.1µg/ml.

    参考文献 (19)

    ab9693 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab9693 は 19 報の論文で使用されています。

    • Huang YH  et al. CREBBP/EP300 mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumor-associated macrophage polarization via FBXW7-NOTCH-CCL2/CSF1 axis. Signal Transduct Target Ther 6:10 (2021). PubMed: 33431788
    • Liu D  et al. CSF1-associated decrease in endometrial macrophages may contribute to Asherman's syndrome. Am J Reprod Immunol 83:e13191 (2020). PubMed: 31536655
    • Sidorkiewicz I  et al. Plasma Levels and Tissue Expression of Selected Cytokines, Metalloproteinases and Tissue Inhibitors in Patients With Cervical Cancer. Anticancer Res 39:6403-6412 (2019). PubMed: 31704874
    • Chen X  et al. CircRNA_28313/miR-195a/CSF1 axis modulates osteoclast differentiation to affect OVX-induced bone absorption in mice. RNA Biol 16:1249-1262 (2019). PubMed: 31204558
    • Zhen R  et al. Hepatocyte growth factor improves bone regeneration via the bone morphogenetic protein-2-mediated NF-?B signaling pathway. Mol Med Rep 17:6045-6053 (2018). PubMed: 29436622
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-MCSF antibody

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Kidney transfected with LFABP)
    Loading amount
    15 µg
    Specification
    Kidney transfected with LFABP
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Miss. Valentina Marcelli

    Verified customer

    投稿 Jun 11 2010

    Question

    Need a product which will work in functional assay and recognize mouse.

    Read More
    Answer

    Thank you for contacting Abcam.

    We currently only have one product which is guaranteed to work in mouse, however, as we have spoken about, this product is currently unavailable in the US.

    We do have two other products which have been shown to work in neutralizing assays. These are the rabbit polyclonal antibody ab9693 (https://www.abcam.com/MCSF-antibody-ab9693.html) and a mouse monoclonal, ab89571 (https://www.abcam.com/MCSF-antibody-ab9693.html).

    I was able to aquire the immunogen sequence used for Ab9693. This sequence had an 80% identity and 91% positive against the related mouse protein. The polyclonal and would recognize multiple epitope sites within this region as compared to a monoclonal which would only recognize one site which may not be present in mouse protein. As such I would recommend ab9693 for your studies.



    To our knowledge, ab9693 has not been tested in mouse. Therefore, I can offer a discount off a future purchase if you buy ab9693 now, test it imouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab9693 in mouse. I will then send a discount code. This code must be issued before purchase so please wait for my reply before ordering.

    2. Purchase either by phone, fax, or online (www.abcam.com).

    3. Test it in mouse.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

    If this rabbit polyclonal does not suit your needs I may also offer this program for testing of ab89571.Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com