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リコンビナントRabMAb

Anti-LYVE1 抗体 [EPR21771] (ab218535)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
  • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
  • Western blot - Anti-LYVE1 antibody [EPR21771] (ab218535)
  • Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] (ab218535)
  • Flow Cytometry - Anti-LYVE1 antibody [EPR21771] (ab218535)
  • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
  • Anti-LYVE1 antibody [EPR21771] (ab218535)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21771] to LYVE1
  • Suitable for: IP, WB, IHC-P, IHC-Fr, Flow Cyt
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 APC Carrier Free

製品の概要

  • 製品名

    Anti-LYVE1 antibody [EPR21771]
    LYVE1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR21771] to LYVE1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WB, IHC-P, IHC-Fr, Flow Cytmore details

  • 種交差性

    交差種: Mouse

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse lymph node and lung lysates; bEnd.3 whole cell lysate. IHC-P: Mouse liver, lung and colon tissues. IHC-Fr: Mouse liver and stomach tissues. Flow Cyt: bEnd.3 cells. IP: Mouse lung lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR21771

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab218535の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/30.
WB
1/1000. Detects a band of approximately 34-70 kDa (predicted molecular weight: 35 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
1/60.
特記事項
IP
1/30.
WB
1/1000. Detects a band of approximately 34-70 kDa (predicted molecular weight: 35 kDa).
IHC-P
1/5000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/500.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt
1/60.

ターゲット情報

  • 機能

    Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

  • 組織特異性

    Mainly expressed in endothelial cells lining lymphatic vessels.

  • 配列類似性

    Contains 1 Link domain.

  • 翻訳後修飾

    O-glycosylated.

  • 細胞内局在

    Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Cell surface retention sequence-binding protein 1 antibody
      • CRSBP 1 antibody
      • CRSBP-1 antibody
      • CRSBP1 antibody
      • extracellular link domain containing 1 antibody
      • extracellular link domain-containing 1 antibody
      • Extracellular link domain-containing protein 1 antibody
      • HAR antibody
      • Hyaluronic acid receptor antibody
      • Lymphatic endothelium specific hyaluronan receptor antibody
      • lymphatic vessel endothelial hyaluronan receptor 1 antibody
      • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
      • LYVE 1 antibody
      • LYVE-1 antibody
      • LYVE1 antibody
      • LYVE1_HUMAN antibody
      • XLKD1 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

      Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling LYVE1 with ab218535 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the lymphatic endothelial cells of mouse lung is observed. Counter stained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
      Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse stomach tissue labeling LYVE1 with ab218535 at 1/5000 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining of the endothelium of lymph vessels in the submucosae on mouse stomach tissue section (PMID: 15705793).

      The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

      Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling LYVE1 with ab218535 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the lymphatic endothelium of mouse colon (PMID: 14722766). Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Western blot - Anti-LYVE1 antibody [EPR21771] (ab218535)
      Western blot - Anti-LYVE1 antibody [EPR21771] (ab218535)
      All lanes : Anti-LYVE1 antibody [EPR21771] (ab218535) at 1/1000 dilution

      Lane 1 : Mouse lymph node lysate
      Lane 2 : bEnd.3 (mouse brain endothelioma cell line) whole cell lysate
      Lane 3 : Mouse lung lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 35 kDa
      Observed band size: 34-70 kDa why is the actual band size different from the predicted?



       

      Exposure time : Lane 1: 3 minutes; Lane 2: 32 seconds; Lane 3: 5 seconds.

      Blocking/Dilution buffer: 5% NFDM/TBST.

      Several bands are observed including soluble, glycosylated and non-glycosylated forms which are consistent with the literature (PMID: 26966180).

    • Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] (ab218535)
      Immunoprecipitation - Anti-LYVE1 antibody [EPR21771] (ab218535)

      LYVE1 was immunoprecipitated from 0.35 mg mouse lung lysate with ab218535 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218535 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

      Lane 1: Mouse lung lysate 10 µg (Input).

      Lane 2: ab218535 IP in mouse lung lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218535 in mouse lung lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 8 seconds.

      Several bands are observed including soluble, glycosylated and non-glycosylated forms which are consistent with the literature (PMID: 26966180).

    • Flow Cytometry - Anti-LYVE1 antibody [EPR21771] (ab218535)
      Flow Cytometry - Anti-LYVE1 antibody [EPR21771] (ab218535)

      Flow cytometric analysis of bEnd.3 (mouse brain endothelioma cell line) cells labeling LYVE1 with ab218535 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

      Gated on total viable cells.

    • Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
      Immunohistochemistry (Frozen sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

      Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling LYVE1 with ab218535 at 1/500 dilution (green), followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Positive staining of the endothelium of sinusoid blood vessels on mouse liver tissue section (PMID: 11719431).

      The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody [EPR21771] (ab218535)

      Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling LYVE1 with ab218535 at 1/5000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on the endothelial surface of mouse hepatic sinusoids (PMID: 16353487; PMID: 11719431). Counter stained with Hematoxylin. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    • Anti-LYVE1 antibody [EPR21771] (ab218535)
      Anti-LYVE1 antibody [EPR21771] (ab218535)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (1)

    ab218535 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab218535 は 1 報の論文で使用されています。

    • Hunt NJ  et al. Quantum Dot Nanomedicine Formulations Dramatically Improve Pharmacological Properties and Alter Uptake Pathways of Metformin and Nicotinamide Mononucleotide in Aging Mice. ACS Nano 15:4710-4727 (2021). PubMed: 33626869

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