Application
Western blot
Sample
Human Cell lysate - whole cell (lung and nasal tissue)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-16%)
Loading amount
30 µg
Specification
lung and nasal tissue
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Concentration
1 µg/ml
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti-rattit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemiluminescence
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 35 kDa
Additional data
Additional Notes
This is a good antibody. We used this antibody for WB and it worked beautifull. Strong, clear and specific band.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 16 2019