Anti-LYVE1 抗体 - BSA and Azide free (ab14917)
Key features and details
- Rabbit polyclonal to LYVE1 - BSA and Azide free
- Suitable for: ICC, IHC-P
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LYVE1 antibody - BSA and Azide free
LYVE1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LYVE1 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IHC-Pmore details -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment (His-tag) corresponding to Mouse LYVE1 aa 1-250 (C terminal).
Database link: Q8BHC0 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Protein A purified -
特記事項（精製）
Protein-A Chromatography (+his tag depleted). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14917の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
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| IHC-P | (27) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ICC
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes. -
組織特異性
Mainly expressed in endothelial cells lining lymphatic vessels. -
配列類似性
Contains 1 Link domain. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface. - Information by UniProt
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参照データベース
- Entrez Gene: 114332 Mouse
- SwissProt: Q8BHC0 Mouse
- Unigene: 396078 Mouse
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別名
- Cell surface retention sequence-binding protein 1 antibody
- CRSBP 1 antibody
- CRSBP-1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)
Immunohistochemical analysis of paraffin-embedded mouse intestine tissue staining LYVE-1 with ab14917. Positive staining is shown in the lymphatic endothelial cells (red).
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Immunocytochemistry - Anti-LYVE1 antibody - BSA and Azide free (ab14917)Immunocytochemistry/immunofluorecent analysis of mouse colon tissue labelling LYVE-1 with ab14917 (red).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)Divan et al PLoS One. 2018 May 3;13(5):e0196893. doi: 10.1371/journal.pone.0196893. eCollection 2018. Fig S1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/At the time point of 75dpi spirochetes (Bb) were not observed in association with the lymphatic-like vessels (LYVE-1) that run parallel to the sagittal sinus (SS, arrow) of the dura mater.
Dura samples were collected from transcardially perfused mice by craniotomy and post-fixed in 4% paraformaldehyde for 24h at 4°C. Samples were permeabilized in 0.1% Triton X-100, washed 3 times, and serum-blocked in 2.5% goat serum/PBS containing 1:100 dilution of Fc block. For B. burgdorferi staining, each sample was incubated in 1:100 dilution of rat anti-mouse unconjugated monoclonal anti-CD31 IgG, and 1:50 dilution biotinylated rabbit anti-B. burgdorferi polyclonal IgG at 4°C overnight. On the following day, the samples were washed, and stained with 1:100 dilution of Alexa 555 goat anti-rat polyclonal IgG, and 1:200 dilution of Alexa 488 streptavidin for 1 hour at room temperature, covered from light. Secondary antibody-only controls for B. burgdorferi indirect fluorescent assay were performed in vitro and no fluorescence was observed.
Some of the dura samples were also stained for lymphatic vessels in a separate step, using 1:200 ab14917, followed by washing and secondary staining with 1:200 Alexa 633 goat-anti rabbit polyclonal IgG (yellow).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)This image is courtesy of an Abreview submitted by Elizabeth Chlipala on 18 November 2005.ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)Image is courtesy of an anonymous Abreviewab14917 staining LYVE1 in mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a EDTA-buffer pH 9.0. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 20°C. A biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYVE1 antibody - BSA and Azide free (ab14917)This image is courtesy of an anonymous Abreviewab14917 at 1/2000 dilution staining Ha-Ras transgenic mouse bladder (cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the primary antibody for 12 hours. A biotinylated polyclonal antibody was used as the secondary.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (220)
ab14917 は 220 報の論文で使用されています。
- Huo Q et al. Free CA125 promotes ovarian cancer cell migration and tumor metastasis by binding Mesothelin to reduce DKK1 expression and activate the SGK3/FOXO3 pathway. Int J Biol Sci 17:574-588 (2021). PubMed: 33613114
- Chang TM et al. c-Myc promotes lymphatic metastasis of pancreatic neuroendocrine tumor through VEGFC upregulation. Cancer Sci 112:243-253 (2021). PubMed: 33128283
- Bae MG et al. Effects of 6,8-Diprenylgenistein on VEGF-A-Induced Lymphangiogenesis and Lymph Node Metastasis in an Oral Cancer Sentinel Lymph Node Animal Model. Int J Mol Sci 22:N/A (2021). PubMed: 33466636
- Wang J et al. Overexpression Prox1 in HemECs resembles Kaposiform hemangioendothelioma and cytotoxicity of sirolimus in vitro. J Pediatr Surg 56:1203-1210 (2021). PubMed: 33865602
- Liang Q et al. Lymphatic muscle cells contribute to dysfunction of the synovial lymphatic system in inflammatory arthritis in mice. Arthritis Res Ther 23:58 (2021). PubMed: 33602317