Anti-Lysozyme 抗体 [SP350] - BSA and Azide free (ab242430)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP350] to Lysozyme - BSA and Azide free
- Suitable for: Flow Cyt (Intra), mIHC, WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Lysozyme antibody [SP350] - BSA and Azide free
Lysozyme 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP350] to Lysozyme - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), mIHC, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human Lysozyme (C terminal). The exact sequence is proprietary.
Database link: P61626 -
ポジティブ・コントロール
- WB: HAP1, HepG2 and HL-60 whole cell lysate. IHC-P: Human tonsil, lung, liver, spleen, stomach adenocarcinoma and pancreatic adenocarcinoma tissues. Flow Cyt (intra): HL-60 cells. mIHC: Human duodenum tissue.
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特記事項
ab242430 is the carrier-free version of ab227708.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.20
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP350 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab242430の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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mIHC |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa.
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IHC-P |
Use at an assay dependent concentration.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol. |
特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
mIHC
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 16 kDa. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 8.0 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. -
関連疾患
Amyloidosis 8 -
配列類似性
Belongs to the glycosyl hydrolase 22 family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- Unigene: 524579 Human
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別名
- 1 4 beta N acetylmuramidase C antibody
- 1 antibody
- 4-beta-N-acetylmuramidase C antibody
see all
画像
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Fluorescence multiplex immunohistochemical analysis of the human duodenum (Formalin/PFA-fixed paraffin-embedded sections). Panel A: merged staining of anti-liver FABP (ab240401, gray; Opal™690), anti-Lysozyme (ab242430, green; Opal™520) and anti-Chromogranin A (ab254557, red; Opal™570) on human duodenum. Panel B: anti-liver FABP stained on enterocytes. Panel C: anti-Lysozyme stained on Paneth cells. Panel D: anti-Chromogranin A stained on neuroendocrine cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of ab240401 (1/8000 dilution), ab242430 (1:250 dilution), and ab254557 (1/5000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using the same antibody clone in a different buffer formulation (ab227708).
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All lanes : Anti-Lysozyme antibody [SP350] - C-terminal (ab227708) at 1/400 dilution
Lane 1 : Wild-type THP1 cell lysate
Lane 2 : LYZ knockout THP1 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 16 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab227708).
Lanes 1 - 4: Merged signal (red and green). Green - ab227708 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab227708 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab227708 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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Intracellular flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab227708 at 1/100 dilution (green) compared with a Rabbit IgG negative control (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA,and sodium azide (ab227708).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab242430 は論文での使用が確認できていません。