Anti-LYRIC/AEG1 抗体 (ab45338)
Key features and details
- Rabbit polyclonal to LYRIC/AEG1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-LYRIC/AEG1 antibody
LYRIC/AEG1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LYRIC/AEG1 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- This antibody gave a positive signal in mouse and rat skeletal muscle tissue lysates, and in mouse heart tissue lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human skin cancer. ICC/IF: HeLa and MCF7 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45338の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use a concentration of 5 - 10 µg/ml.
Suitable for use in cells fixed with either 100% methanol (5mins) or 4% PFA (10mins). |
WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
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IHC-P |
Use a concentration of 5 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 5 - 10 µg/ml. Suitable for use in cells fixed with either 100% methanol (5mins) or 4% PFA (10mins). |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa). |
IHC-P
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance. -
組織特異性
Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme. -
細胞内局在
Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 509788 Cow
- Entrez Gene: 92140 Human
- Entrez Gene: 67154 Mouse
- Entrez Gene: 170910 Rat
- Omim: 610323 Human
- SwissProt: Q86UE4 Human
- SwissProt: Q80WJ7 Mouse
- SwissProt: Q9Z1W6 Rat
see all -
別名
- 3D3 antibody
- 3D3/LYRIC antibody
- AEG 1 antibody
see all
画像
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ab45338 staining LYRIC in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1%PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab45338 at 10μg/ml and ab7291, Anti-alpha Tubulin antibody [DM1A] - Loading Control, at 1/1000 dilution. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed (shown in green) and ab1500120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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All lanes : Anti-LYRIC/AEG1 antibody (ab45338) at 1 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 3 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : DU145 (Human Prostate carcinoma epithelial like cell line) Whole Cell lysate
Lane 5 : PC-3 whole cell lysate (ab3954)
Lane 6 : U-87 MG nuclear extract lysate (ab14903)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 64 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 80 kDa (possible post-translational modification). We are unsure as to the identity of these extra bands. -
All lanes : Anti-LYRIC/AEG1 antibody (ab45338) at 1 µg/ml
Lane 1 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Skeletal Muscle (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 64 kDa
Exposure time: 1 minute -
IHC image of LYRIC/AEG1 staining in Human skin cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45338, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times
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ICC/IF image of ab45338 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45338, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells fixed in 100% methanol (10 min) cells.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (19)
ab45338 は 19 報の論文で使用されています。
- Wang Y et al. MiR-9-3p regulates the biological functions and drug resistance of gemcitabine-treated breast cancer cells and affects tumor growth through targeting MTDH. Cell Death Dis 12:861 (2021). PubMed: 34552061
- Guo R et al. Long non-coding RNA PRNCR1 modulates non-small cell lung cancer cell proliferation, apoptosis, migration, invasion, and EMT through PRNCR1/miR-126-5p/MTDH axis. Biosci Rep 40:N/A (2020). PubMed: 31912882
- Zhang H et al. CPEB3-mediated MTDH mRNA translational suppression restrains hepatocellular carcinoma progression. Cell Death Dis 11:792 (2020). PubMed: 32968053
- Jiao Y et al. miR-3664-5P suppresses the proliferation and metastasis of gastric cancer by attenuating the NF-?B signaling pathway through targeting MTDH. Int J Oncol 54:845-858 (2019). PubMed: 30628643
- Peng Y et al. Silencing astrocyte elevated gene-1 attenuates lipopolysaccharide-induced inflammation and mucosal barrier injury in NCM460 cells by suppressing the activation of NLRP3 inflammasome. Cell Biol Int 43:56-64 (2019). PubMed: 30489008