Western blot abreview for Anti-Lyn antibody [LYN-01]

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (bone marrow derived mast cells)
Loading amount
10 µg
Specification
bone marrow derived mast cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA-TBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-mouse IgG HRP linked
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 56, 53 kDa

Additional data

Additional Notes
Lanes are whole cell lysates in RIPA buffer, with protease and phosphatase inhibitors, of BMMCs from WT and Itk-/- mice stimulated with IgE + DNP-HSA for 0, 2, 5, or 15 minutes.

Mr. Stefan Schattgen

Verified customer

投稿 Sep 25 2007

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