Application
Western blot
Sample
Human Cell lysate - whole cell (Platelet Lysate (precleared))
Loading amount
25 µg
Specification
Platelet Lysate (precleared)
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Starting Block - Thermo as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 2%BSA
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-mouse HRP conjugated
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 48 kDa
Additional data
Additional Notes
Starting Block is a commercially available blocking buffer (1X) which was used undiluted.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Mahesh Shivananjappa
Verified customer
投稿 Jan 14 2010