Anti-Ly6c 抗体 [HK1.4] (ab24973)
Key features and details
- Rat monoclonal [HK1.4] to Ly6c
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2c
Related conjugates and formulations
製品の概要
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製品名
Anti-Ly6c antibody [HK1.4]
Ly6c 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [HK1.4] to Ly6c -
由来種
Rat -
アプリケーション
適用あり: Flow Cytmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Ly6c. L3 cloned CTL cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 8.2 -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
モノクローナル -
クローン名
HK1.4 -
アイソタイプ
IgG2c -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab24973の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
特記事項 |
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Flow Cyt: Use 1µg for 106 cells.
IHC-Fr: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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関連性
Ly6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is a member of the Ly6 multigene family of type V glycophosphatidylinositol anchored cell surface proteins. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Mice with the Ly6.2 allotype (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have subsets of CD4+Ly6C+ and CD8+Ly6C+ cells, while Ly6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have only CD8+Ly6C+ lymphocytes. -
細胞内局在
Cell membrane; Lipid-anchor, GPI-anchor. -
参照データベース
- Entrez Gene: 17067 Mouse
- SwissProt: P0CW02 Mouse
- SwissProt: P0CW03 Mouse
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別名
- Ly 6c antibody
- Ly6c protein antibody
- Ly6c1 antibody
see all
画像
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab24973 は 6 報の論文で使用されています。
- Zhang X & Dong S Protective effect of growth differentiation factor 15 in sepsis by regulating macrophage polarization and its mechanism. Bioengineered 13:9687-9707 (2022). PubMed: 35420978
- Feng Z et al. Ly6G+ neutrophil-derived miR-223 inhibits the NLRP3 inflammasome in mitochondrial DAMP-induced acute lung injury. Cell Death Dis 8:e3170 (2017). PubMed: 29144508
- Liyanage SE et al. Flow cytometric analysis of inflammatory and resident myeloid populations in mouse ocular inflammatory models. Exp Eye Res 151:160-70 (2016). IHC-P . PubMed: 27544307
- Zhao C et al. Nuclear Factor E2-related Factor-2 (Nrf2) Is Required for NLRP3 and AIM2 Inflammasome Activation. J Biol Chem 289:17020-17029 (2014). Flow Cyt . PubMed: 24798340
- Scindia Y et al. Activated CD4+ T cells target mesangial antigens and initiate glomerulonephritis. Nephron Exp Nephrol 121:e1-9 (2012). PubMed: 23095207
- Makaroff LE et al. Postthymic maturation influences the CD8 T cell response to antigen. Proc Natl Acad Sci U S A 106:4799-804 (2009). Flow Cyt ; Mouse . PubMed: 19270077