Application
Western blot
Sample
Rat Tissue lysate - whole (LIVER)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s)
Concentration
2 µg/ml
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Santacruz
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
Additional data
Additional Notes
Liver tissue were homogenized with RIPA buffer (RIPA buffer pH 8.6 containing 150 mM NaCI, 1% NP — 40, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, 5 mM EDTA, 1 mM EGTA, 10 mM sodium fluoride, 20 mM DTT, 1 mM benzamidine HCI, 1mMPMSF, 1 µM aprotinin, pepstatin A and okadaic acid,
All lanes: Liver lysates L1,2= rat with normal chow. L3,4= rat treated with High fat diet. L5-8: rat treated with normal chow and fat along with treatment group
Nitrocellulose membrane and CL – Xposure films were from Amersham
ECL: WestPico Super Signal
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Oct 11 2016