Anti-LSECtin 抗体 [EPR13724] (ab181196)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13724] to LSECtin
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-LSECtin antibody [EPR13724]
LSECtin 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR13724] to LSECtin -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- WB: Human fetal liver lysate and mouse liver lysates. IHC-P: Human lymph node lysate and tissue, Human liver tissue.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR13724 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab181196の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa).
|
|
IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
特記事項 |
---|
WB
1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 33 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ターゲット情報
-
配列類似性
Contains 1 C-type lectin domain. -
細胞内局在
Membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 339390 Human
- Entrez Gene: 75863 Mouse
- Omim: 616256 Human
- SwissProt: Q6UXB4 Human
- SwissProt: Q8BNX1 Mouse
- Unigene: 220649 Human
- Unigene: 109183 Mouse
-
別名
- C type lectin domain family 4, member G antibody
- C type lectin superfamily 4, member G antibody
- C-type lectin domain family 4 member G antibody
see all
画像
-
Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution + Mouse liver tissue lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 33 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 180 seconds -
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LSECtin with ab181196 at 1/250 dilution. Secondary antibody: anti rabbit IgG (HRP) prediluted. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
All lanes : Anti-LSECtin antibody [EPR13724] (ab181196) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human lymph node lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 33 kDa
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (2)
ab181196 は 2 報の論文で使用されています。
- Fang S et al. CXCL8 Up-Regulated LSECtin through AKT Signal and Correlates with the Immune Microenvironment Modulation in Colon Cancer. Cancers (Basel) 14:N/A (2022). PubMed: 36358719
- Wang JB et al. An immune checkpoint score system for prognostic evaluation and adjuvant chemotherapy selection in gastric cancer. Nat Commun 11:6352 (2020). PubMed: 33311518