Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online

Anti-LRP1 抗体 [8G1] (ab20384)

Reviews (2)Q&A (4)References (13)
Immunocytochemistry/ Immunofluorescence - Anti-LRP1 antibody [8G1] (ab20384)
  • Electron Microscopy - Anti-LRP1 antibody [8G1] (ab20384)

Key features and details

  • Mouse monoclonal [8G1] to LRP1
  • Suitable for: ICC/IF, Electron Microscopy
  • Reacts with: Rat, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-LRP1 antibody [8G1]
    LRP1 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [8G1] to LRP1

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, Electron Microscopymore details

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Full length protein corresponding to LRP1. J Biol Chem 265:17401-4 (1990).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab20384の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC/IF (1)
Use a concentration of 5 µg/ml.
Electron Microscopy
Use at an assay dependent concentration. Use at dilutions of 1/400 or 1/600 for 20 mins.
特記事項
ICC/IF
Use a concentration of 5 µg/ml.
Electron Microscopy
Use at an assay dependent concentration. Use at dilutions of 1/400 or 1/600 for 20 mins.

ターゲット情報

  • 機能

    Endocytic receptor involved in endocytosis and in phagocytosis of apoptotic cells. Required for early embryonic development. Involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission.
    Functions as a receptor for Pseudomonas aeruginosa exotoxin A.

  • 組織特異性

    Most abundant in liver, brain and lung.

  • 配列類似性

    Belongs to the LDLR family.
    Contains 22 EGF-like domains.
    Contains 31 LDL-receptor class A domains.
    Contains 34 LDL-receptor class B repeats.

  • 翻訳後修飾

    Cleaved into a 85 kDa membrane-spanning subunit (LRP-85) and a 515 kDa large extracellular domain (LRP-515) that remains non-covalently associated. Gamma-secretase-dependent cleavage of LRP-85 releases the intracellular domain from the membrane.
    The N-terminus is blocked.
    Phosphorylated on serine and threonine residues.
    Phosphorylated on tyrosine residues upon stimulation with PDGF. Tyrosine phosphorylation promotes interaction with SHC1.

  • 細胞内局在

    Cytoplasm. Nucleus. After cleavage, the intracellular domain (LRPICD) is detected both in the cytoplasm and in the nucleus and Cell membrane. Membrane, coated pit.
  • Information by UniProt

  • 参照データベース

    • 別名

      • A2MR antibody
      • Alpha 2 macroglobulin receptor antibody
      • alpha 2MR antibody
      • Alpha-2-macroglobulin receptor antibody
      • APOER antibody
      • Apolipoprotein E receptor antibody
      • APR antibody
      • CD 91 antibody
      • CD91 antibody
      • CD91 antigen antibody
      • IGFBP3R antibody
      • LDL receptor related protein 1 antibody
      • Low density lipoprotein receptor related protein 1 antibody
      • Low density lipoprotein related protein 1 antibody
      • Low-density lipoprotein receptor-related protein 1 intracellular domain antibody
      • LRP 1 antibody
      • LRP 515 antibody
      • LRP 85 antibody
      • LRP antibody
      • LRP ICD antibody
      • LRP-1 antibody
      • LRP-515 antibody
      • LRP-85 antibody
      • Lrp1 antibody
      • LRP1 protein antibody
      • LRP1_HUMAN antibody
      • LRP1A antibody
      • LRP515 antibody
      • LRP85 antibody
      • LRPICD antibody
      • MGC88725 antibody
      • Prolow density lipoprotein receptor related protein 1 antibody
      • TbetaR V/LRP 1/IGFBP 3 receptor antibody
      • TbetaRV/LRP1/IGFBP3 receptor antibody
      • TGFBR 5 antibody
      • TGFBR5 antibody
      • Type V tgf beta receptor antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-LRP1 antibody [8G1] (ab20384)
      Immunocytochemistry/ Immunofluorescence - Anti-LRP1 antibody [8G1] (ab20384)
      ICC/IF image of ab20384 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20384, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Electron Microscopy - Anti-LRP1 antibody [8G1] (ab20384)
      Electron Microscopy - Anti-LRP1 antibody [8G1] (ab20384)
      Ab20384 staining primary rat hepatocytes cells by EM. The cells were fixed and prepared by Tokuyasu method. The cryosections of 70nm were placed on 100 mesh grids. All the following steps were done at room temperature. Blocking was carried out for 30 min with 1% fish skin gelatine (FSG). The tissue sections were placed on a drop of 5 ul of first Ab (anti-LRP 8G1) for 20 min at dilutions of 1:400 or 1:600 seemed to work the best. After incubation with the primary antibody, 3. 5 x 2 min wash was done with PBS and the sections were placed on a drop of 5 ul of secondary Ab (goat anti-mouse) for 20 min (dilution 1:150 5. 5 x 2 min wash with PBS). Samples were placed on a drop of 5 ul of PAG10 for 15 min (dilution 1:60 or 1:70) and then 5 x 2 min wash with PBS. 2 min on 1% glutaraldehyde for fixation and 4 x 2.5 min on H2O. afterthat 1 second + 1 second + 6 min on drops of Methyl Cellulose/Uranyl Acetate (9:1) on ice. The surplus of MC/UA was removed , the slides were dried and obser

    参考文献 (13)

    ab20384 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab20384 は 13 報の論文で使用されています。

    • Remacle AG  et al. Interaction of the cryptic fragment of myelin basic protein with mitochondrial voltage-dependent anion-selective channel-1 affects cell energy metabolism. Biochem J 475:2355-2376 (2018). PubMed: 29954845
    • Chan CY  et al. Clearance of matrix metalloproteinase-9 is dependent on low-density lipoprotein receptor-related protein-1 expression downregulated by microRNA-205 in human abdominal aortic aneurysm. J Vasc Surg 65:509-520 (2017). PubMed: 26781079
    • Boyé K  et al. The role of CXCR3/LRP1 cross-talk in the invasion of primary brain tumors. Nat Commun 8:1571 (2017). PubMed: 29146996
    • Chanalaris A  et al. Suramin Inhibits Osteoarthritic Cartilage Degradation by Increasing Extracellular Levels of Chondroprotective Tissue Inhibitor of Metalloproteinases 3. Mol Pharmacol 92:459-468 (2017). PubMed: 28798097
    • Wilkinson DJ  et al. Matriptase Induction of Metalloproteinase-Dependent Aggrecanolysis In Vitro and In Vivo: Promotion of Osteoarthritic Cartilage Damage by Multiple Mechanisms. Arthritis Rheumatol 69:1601-1611 (2017). PubMed: 28464560
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-LRP1 antibody [8G1]

     Poor
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (hepatocyte)
    Permeabilization
    No
    Specification
    hepatocyte
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 10 2018

    Flow Cytometry abreview for Anti-LRP1 antibody [8G1]

     Good
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Mesothelial Cels)
    Specification
    Mesothelial Cels
    Fixation
    Formaldehyde
    Permeabilization
    No
    Gating Strategy
    gated against mIgG control
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 04 2008

    Question


    Would you mind checking your LRP1 antibodies as well? In my last blast
    search, C. elegans LRP-2 shared the most identity with human LRP-1
    (yes, the names are confusing!). If the human LRP-1 antibodies are a
    no go, I might try the one you suggested for human LRP-2.

    Read More
    Answer

    I checked the LRP1 antibodies and ab20384 has 93% homology with C. elegans.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Customer decided to buy another product ab92544. So we will order it and I will be grateful if you send us credit note.

    Thank you in advance.

    Read More
    Answer

    Thank you for confirming this information and for your help and cooperation with this case.

    As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order.

    Credit ID: ####


    As usual if you have any further questions regarding this credit note, please contact the accounts department by email at creditcontrol@abcam.com. Please refer to the credit ID number in any correspondence with the accounting department.

    I would like to wish the customer good luck with their research. The technical team is always at your service, should you require further expert advice.

    Read More

    Question

    My customer did not consent to giving you her data, I suppose that she afraid to talk in English, she may not feel confident but look at her answers/more details below:

    ab 20384

    lot. GR76320-3

    Staining Protocol:
    1.deparaffining scrap-xylene, alcohol, H2O
    2 - Reveal antigen; a) hot - citrate buffer pH 6-20 minutes
    b) hot-EDTA, pH -9
    c) the enzyme - trypsin-Sigma
    3 - Cooling the room at temperatures - 20 minutes
    4-Methanol + H2O2 - 10 minutes
    5 - Tris buffer pH 7, 6 - 10 min
    6 - Blocking serum (NCL-H-SERUM)-Leica-20 min.
    7 - the primary antibody ab 20 384 - 5ug / 1 ml, room temperature overnight and humid conditions
    8-Tris buffer pH 7, 6 - 10 min
    9 - Secondary Antibody Anti-Mouse IgG-Sigma - B 7151 (1:200) - 45 min
    10-Tris buffer - 10 min
    11 - Streptavidin-HRP Conjugate Millipore (1:500) 60 min
    12-Tris buffer, 10 min
    13 - DAB - 1 to 3 min
    14 - Hematoxylin - coloring
    15 - Dehydration and seal in histofluid.

    The problem is the lack of an immune response each time revealing.
    The positive control is the human tissue in Alzheimer's patients.
    Reagents that were used for staining routinely used for staining with other antibodies without problems

    Read More
    Answer

    Thank you for taking the time to provide the further information.

    The details you have kindly provided will provide us with vital information for our monitoring of product quality

    I appreciate the time the customer has spent in the laboratory and understand your concerns. It is regrettable the results have not been successful. Reviewing the details, I am sorry there are no further tips to provide on this occasion to help improve the results. I can suggest you have regrettably received a bad vial. I apologize for the inconvenience.

    I have also spoken to our distributors team and I am happy to let you know we are prepared to make an exception and continue to provide our Abpromise for Symbios customers. Therefore, I am pleased to offer you a free of charge replacement or credit note in compensation for this case.

    In addition, I can suggest the customer may like to consider the following suggestions as a check for future experiments:

    1. I can suggest to try adding 0.2% Tween to the antibody dilution buffer and wash buffer. This will help to solubilise the antibody and keep the cells permeabilised.

    2. I can recommend to try wash steps: 3 X 5 minutes in PBS containing 0.2% Tween.

    3. We recommend to incubate at 4oC overnight with antibodies. This can often provide more efficient and specific staining.

    4. Is the current vial of secondary antibody working well with other primary antibodies?

    I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Question

    I'm looking for a negative control cell line or tissue type to run with this antibody.

    Read More
    Answer

    Thank you for contacting us.

    For ab20384, the lab recommends using human umbilical vein endothelial cells (HUVEC) as a negative control. If you do not have access to this type of cells, according to the human protein atlas (linked below),Low Density LRP is not expressed in tonsil, skin, or breast tissues, so any of these could be used as a negative control. If you are looking for lysates, you could try ab30090, ab30166, or ab29614 and forslides, you could try ab4365.

    http://www.proteinatlas.org/ENSG00000123384/normal

    I hope this helps, please let me know if you need any additional information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com