Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6478] to LPP - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-LPP antibody [EPR6478] - BSA and Azide free
LPP 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR6478] to LPP - BSA and Azide free
アプリケーション適用あり: IHC-P, WBmore details
適用なし: Flow Cyt or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human LPP aa 1-100 (N terminal). The exact sequence is proprietary.
Ab248124 is the carrier-free version of ab126608. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248124 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 2.60 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 66 kDa).|
機能May play a structural role at sites of cell adhesion in maintaining cell shape and motility. In addition to these structural functions, it may also be implicated in signaling events and activation of gene transcription. May be involved in signal transduction from cell adhesion sites to the nucleus allowing successful integration of signals arising from soluble factors and cell-cell adhesion sites. Also suggested to serve as a scaffold protein upon which distinct protein complexes are assembled in the cytoplasm and in the nucleus.
組織特異性Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes.
関連疾患Note=A chromosomal aberration involving LPP is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with HMGA2 is shown in lipomas.
Note=A chromosomal aberration involving LPP is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with HMGA2 is detected in pulmonary chondroid hamartomas.
Note=A chromosomal aberration involving LPP is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with HMGA2 is also shown in one parosteal lipoma.
Note=A chromosomal aberration involving LPP is associated with acute monoblastic leukemia. Translocation t(3;11)(q28;q23) with MLL is associated with acute monoblastic leukemia.
配列類似性Belongs to the zyxin/ajuba family.
Contains 3 LIM zinc-binding domains.
細胞内局在Nucleus. Cytoplasm. Cell junction. Cell membrane. Found in the nucleus, in the cytoplasm and at cell adhesion sites. Shuttles between the cytoplasm and the nucleus. It has been found in sites of cell adhesion such as cell-to-cell contact and focal adhesion which are membrane attachment sites of cells to the extracellular matrix. Mainly nuclear when fused with HMGA2/HMGIC and MLL.
- Information by UniProt
- DKFZp779O0231 antibody
- FLJ30652 antibody
- FLJ41512 antibody
All lanes : Anti-LPP antibody [EPR6478] (ab126608) at 1/10000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 66 kDa
This data was developed using ab126608, the same antibody clone in a different buffer formulation.
This data was developed using ab126608, the same antibody clone in a different buffer formulation.ab122608 at 1/250 staining LPP in paraffin embedded Human breast tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using ab126608, the same antibody clone in a different buffer formulation.ab122608 at 1/250 staining LPP in paraffin embedded Human kidney tissue by immunohistochemistry. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.