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  1. Link

    liver-fabp-antibody-l2b10-ab7366.pdf

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Signal Transduction Metabolism Lipid metabolism
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Anti-liver FABP 抗体 [L2B10] (ab7366)

  • Datasheet
  • SDS
Reviews (10)Q&A (1)References (14)

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Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)
  • Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)

Key features and details

  • Mouse monoclonal [L2B10] to liver FABP
  • Suitable for: WB, Functional Studies, Flow Cyt, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Cow, Human, African green monkey
  • Isotype: IgG1

こちらの製品もご検討ください

一次抗体
Product image
Anti-BRG1 antibody [EPNCIR111A] (ab110641)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-liver FABP antibody [L2B10]
    liver FABP 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [L2B10] to liver FABP
  • 由来種

    Mouse
  • 特異性

    This monoclonal antibody binds to human liver FABP of both natural and recombinant origin. No reactivity to human intestinal or heart FABP.
  • アプリケーション

    適用あり: WB, Functional Studies, Flow Cyt, IHC-P, IHC-Frmore details
    適用なし: ICC/IF
  • 種交差性

    交差種: Mouse, Rat, Cow, Human, African green monkey
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    L2B10
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7366の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (6)
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 18779054
Functional Studies
Use at an assay dependent concentration.

Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant.

Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 18779054
Functional Studies
Use at an assay dependent concentration.

Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant.

Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
  • 配列類似性

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • ドメイン

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession P07148 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2168 Human
    • Entrez Gene: 14080 Mouse
    • Entrez Gene: 24360 Rat
    • Omim: 134650 Human
    • SwissProt: P07148 Human
    • SwissProt: P12710 Mouse
    • SwissProt: P02692 Rat
    • Unigene: 380135 Human
    • Unigene: 22126 Mouse
    • Unigene: 36412 Rat
    see all
  • 別名

    • FABP 1 antibody
    • FABP1 antibody
    • FABPL antibody
    • FABPL_HUMAN antibody
    • Fatty Acid Binding Protein 1 antibody
    • Fatty acid binding protein 1 liver antibody
    • Fatty Acid Binding Protein antibody
    • Fatty acid-binding protein 1 antibody
    • Fatty acid-binding protein antibody
    • Fatty acid-binding protein liver antibody
    • L FABP antibody
    • L-FABP antibody
    • liver antibody
    • Liver-type fatty acid-binding protein antibody
    see all

画像

  • Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)
    Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)

    Immunohistochemical analysis of human L-FABP in frozen human kidney tissue with ab7366 at 1.9 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)

    Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 200X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)

    Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 50X.

  • Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)
    Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)
    Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (14)

ab7366 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab7366 は 14 報の論文で使用されています。

  • Uhlitz F  et al. Mitogen-activated protein kinase activity drives cell trajectories in colorectal cancer. EMBO Mol Med 13:e14123 (2021). PubMed: 34409732
  • Belcastro L  et al. Decreased Fatty Acid Transporter FABP1 and Increased Isoprostanes and Neuroprostanes in the Human Term Placenta: Implications for Inflammation and Birth Weight in Maternal Pre-Gestational Obesity. Nutrients 13:N/A (2021). PubMed: 34444927
  • Niture S  et al. TNFAIP8 regulates autophagy, cell steatosis, and promotes hepatocellular carcinoma cell proliferation. Cell Death Dis 11:178 (2020). PubMed: 32152268
  • Shen XY  et al. Molecular classification of hepatocellular adenoma: A single-center experience. Ann Hepatobiliary Pancreat Surg 23:109-114 (2019). PubMed: 31225410
  • Lyons J  et al. The colonic epithelium plays an active role in promoting colitis by shaping the tissue cytokine profile. PLoS Biol 16:e2002417 (2018). PubMed: 29596476
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 11 Abreviews or Q&A

Western blot abreview for Anti-liver FABP antibody [L2B10]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Tissue lysate - whole (liver tissue lysate)
Loading amount
10 µg
Specification
liver tissue lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 10 2011

Immunocytochemistry/ Immunofluorescence abreview for Anti-liver FABP antibody [L2B10]

Inconclusive
Abreviews
Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (hepatocyte)
Specification
hepatocyte
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 29 2008

Immunocytochemistry/ Immunofluorescence abreview for Anti-liver FABP antibody [L2B10]

Inconclusive
Abreviews
Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (hepatocyte)
Specification
hepatocyte
Fixative
Methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 May 29 2008

Immunocytochemistry/ Immunofluorescence abreview for Anti-liver FABP antibody [L2B10]

Inconclusive
Abreviews
Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (hepatocytes)
Specification
hepatocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton x-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 20 2008

Western blot abreview for Anti-liver FABP antibody [L2B10]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (cos-7)
Loading amount
75 µg
Specification
cos-7
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 18 2008

Western blot abreview for Anti-liver FABP antibody [L2B10]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (hepatocytes)
Loading amount
50 µg
Specification
hepatocytes
Gel Running Conditions
Reduced Non-Denaturing (Native)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 18 2008

Immunocytochemistry/ Immunofluorescence abreview for Anti-liver FABP antibody [L2B10]

Poor
Abreviews
Abreviews
This product is known to not work in this application or species.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (primary hepatocytes)
Specification
primary hepatocytes
Fixative
Methanol
Permeabilization
No
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 21 2007

Western blot abreview for Anti-liver FABP antibody [L2B10]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (liver)
Loading amount
25 µg
Specification
liver
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 07 2007

Western blot abreview for Anti-liver FABP antibody [L2B10]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary Hepatocytes)
Loading amount
25 µg
Specification
Primary Hepatocytes
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 25 2007

Western blot abreview for Anti-liver FABP antibody [L2B10]

Poor
Abreviews
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
50 µg
Specification
liver
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Ying Chen

Verified customer

投稿 Oct 12 2007

1-10 of 11 Abreviews or Q&A

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