Anti-liver FABP 抗体 [L2B10] (ab7366)
![Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.co.jp/ps/products/7/ab7366/Images/ab7366-282298-anti-liver-fabp-antibody-l2b10-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)")
Key features and details
- Mouse monoclonal [L2B10] to liver FABP
- Suitable for: WB, Functional Studies, Flow Cyt, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Cow, Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-liver FABP antibody [L2B10]
liver FABP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [L2B10] to liver FABP -
由来種
Mouse -
特異性
This monoclonal antibody binds to human liver FABP of both natural and recombinant origin. No reactivity to human intestinal or heart FABP. -
アプリケーション
適用あり: WB, Functional Studies, Flow Cyt, IHC-P, IHC-Frmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Cow, Human, African green monkey -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
L2B10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab7366の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (6) |
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 18779054
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| Functional Studies |
Use at an assay dependent concentration.
Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant. |
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IHC-P |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 18779054 |
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Functional Studies
Use at an assay dependent concentration. Liver FABP is a sensitive marker for cell damage of liver cells in vivo and useful for example for in vitro toxicology tests. Cytotoxicity protocol: * Culture 100 ml of 1.105 HUH-7 cells per ml in 96-wells plate. * T=0: Add 100 ml of 2x test chemicals to 96-wells plate. * Incubate 37 degree C, 5% CO2. * T=24: measure L-FABP in supernatant. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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IHC-P
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
ターゲット情報
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機能
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. -
配列類似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
ドメイン
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2168 Human
- Entrez Gene: 14080 Mouse
- Entrez Gene: 24360 Rat
- Omim: 134650 Human
- SwissProt: P07148 Human
- SwissProt: P12710 Mouse
- SwissProt: P02692 Rat
- Unigene: 380135 Human
see all -
別名
- FABP 1 antibody
- FABP1 antibody
- FABPL antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)
Immunohistochemical analysis of human L-FABP in frozen human kidney tissue with ab7366 at 1.9 μg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.co.jp/ps/products/7/ab7366/Images/ab7366-222193-anti-liver-fabp-antibody-l2b10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 200X.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.co.jp/ps/products/7/ab7366/Images/ab7366-222192-anti-liver-fabp-antibody-l2b10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 50X.
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![Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.co.jp/ps/products/7/ab7366/Images/ab7366-12927-anti-liver-fabp-antibody-l2b10-flow-cytometry.jpg)
Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab7366 は 12 報の論文で使用されています。
- Niture S et al. TNFAIP8 regulates autophagy, cell steatosis, and promotes hepatocellular carcinoma cell proliferation. Cell Death Dis 11:178 (2020). PubMed: 32152268
- Shen XY et al. Molecular classification of hepatocellular adenoma: A single-center experience. Ann Hepatobiliary Pancreat Surg 23:109-114 (2019). PubMed: 31225410
- Lyons J et al. The colonic epithelium plays an active role in promoting colitis by shaping the tissue cytokine profile. PLoS Biol 16:e2002417 (2018). PubMed: 29596476
- Takahashi T et al. Histopathological characteristics of glutamine synthetase-positive hepatic tumor lesions in a mouse model of spontaneous metabolic syndrome (TSOD mouse). Mol Clin Oncol 5:267-270 (2016). IHC-P ; Mouse . PubMed: 27446562
- Smathers RL et al. Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver. J Lipid Res 54:1335-45 (2013). WB ; Mouse . PubMed: 23359610