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  1. Link

    liver-fabp-antibody-l2b10-ab7366.pdf

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Signal Transduction Metabolism Lipid metabolism
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Anti-liver FABP 抗体 [L2B10] (ab7366)

  • Datasheet
  • SDS
Reviews (10)Q&A (1)References (12)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)
  • Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)

Key features and details

  • Mouse monoclonal [L2B10] to liver FABP
  • Suitable for: Flow Cyt, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human, African green monkey
  • Isotype: IgG1

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-liver FABP antibody [L2B10]
    liver FABP 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [L2B10] to liver FABP
  • 由来種

    Mouse
  • 特異性

    This monoclonal antibody binds to human liver FABP of both natural and recombinant origin. No reactivity to human intestinal or heart FABP.
  • アプリケーション

    適用あり: Flow Cyt, IHC-P, IHC-Frmore details
    適用なし: ICC/IF
  • 種交差性

    交差種: Mouse, Rat, Human, African green monkey
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...
  • 精製度

    Protein G purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    L2B10
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

アプリケーション

Our Abpromise guarantee covers the use of ab7366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for ICC/IF.
  • ターゲット情報

    • 機能

      Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport.
    • 配列類似性

      Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
    • ドメイン

      Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
    • 細胞内局在

      Cytoplasm.
    • Target information above from: UniProt accession P07148 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 2168 Human
      • Entrez Gene: 14080 Mouse
      • Entrez Gene: 24360 Rat
      • Omim: 134650 Human
      • SwissProt: P07148 Human
      • SwissProt: P12710 Mouse
      • SwissProt: P02692 Rat
      • Unigene: 380135 Human
      • Unigene: 22126 Mouse
      • Unigene: 36412 Rat
      see all
    • 別名

      • FABP 1 antibody
      • FABP1 antibody
      • FABPL antibody
      • FABPL_HUMAN antibody
      • Fatty Acid Binding Protein 1 antibody
      • Fatty acid binding protein 1 liver antibody
      • Fatty Acid Binding Protein antibody
      • Fatty acid-binding protein 1 antibody
      • Fatty acid-binding protein antibody
      • Fatty acid-binding protein liver antibody
      • L FABP antibody
      • L-FABP antibody
      • liver antibody
      • Liver-type fatty acid-binding protein antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)
      Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)

      Immunohistochemical analysis of human L-FABP in frozen human kidney tissue with ab7366 at 1.9 μg/ml.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)

      Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 200X.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)

      Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 50X.

    • Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)
      Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)
      Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    プロトコール

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (12)

    ab7366 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7366 は 12 報の論文で使用されています。

    • Niture S  et al. TNFAIP8 regulates autophagy, cell steatosis, and promotes hepatocellular carcinoma cell proliferation. Cell Death Dis 11:178 (2020). PubMed: 32152268
    • Shen XY  et al. Molecular classification of hepatocellular adenoma: A single-center experience. Ann Hepatobiliary Pancreat Surg 23:109-114 (2019). PubMed: 31225410
    • Lyons J  et al. The colonic epithelium plays an active role in promoting colitis by shaping the tissue cytokine profile. PLoS Biol 16:e2002417 (2018). PubMed: 29596476
    • Takahashi T  et al. Histopathological characteristics of glutamine synthetase-positive hepatic tumor lesions in a mouse model of spontaneous metabolic syndrome (TSOD mouse). Mol Clin Oncol 5:267-270 (2016). IHC-P ; Mouse . PubMed: 27446562
    • Smathers RL  et al. Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver. J Lipid Res 54:1335-45 (2013). WB ; Mouse . PubMed: 23359610
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 11 Abreviews or Q&A

    Western blot abreview for Anti-liver FABP antibody [L2B10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Tissue lysate - whole (liver tissue lysate)
    Loading amount
    10 µg
    Specification
    liver tissue lysate
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 10 2011

    Immunocytochemistry/ Immunofluorescence abreview for Anti-liver FABP antibody [L2B10]

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African Green Monkey Cell (hepatocyte)
    Specification
    hepatocyte
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 29 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-liver FABP antibody [L2B10]

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (hepatocyte)
    Specification
    hepatocyte
    Fixative
    Methanol
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 May 29 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-liver FABP antibody [L2B10]

    Inconclusive
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (hepatocytes)
    Specification
    hepatocytes
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.1% triton x-100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 20 2008

    Western blot abreview for Anti-liver FABP antibody [L2B10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (cos-7)
    Loading amount
    75 µg
    Specification
    cos-7
    Gel Running Conditions
    Reduced Denaturing (15% gel)
    Blocking step
    Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 18 2008

    Western blot abreview for Anti-liver FABP antibody [L2B10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (hepatocytes)
    Loading amount
    50 µg
    Specification
    hepatocytes
    Gel Running Conditions
    Reduced Non-Denaturing (Native)
    Blocking step
    Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 18 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-liver FABP antibody [L2B10]

    Poor
    Abreviews
    Abreviews
    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (primary hepatocytes)
    Specification
    primary hepatocytes
    Fixative
    Methanol
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 21 2007

    Western blot abreview for Anti-liver FABP antibody [L2B10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (liver)
    Loading amount
    25 µg
    Specification
    liver
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 07 2007

    Western blot abreview for Anti-liver FABP antibody [L2B10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Primary Hepatocytes)
    Loading amount
    25 µg
    Specification
    Primary Hepatocytes
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 25 2007

    Western blot abreview for Anti-liver FABP antibody [L2B10]

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (liver)
    Loading amount
    50 µg
    Specification
    liver
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Ying Chen

    Verified customer

    投稿 Oct 12 2007

    1-10 of 11 Abreviews or Q&A

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