Anti-liver FABP 抗体 [L2B10] (ab7366)
![Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.co.jp/ps/products/7/ab7366/Images/ab7366-282298-anti-liver-fabp-antibody-l2b10-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)")
Key features and details
- Mouse monoclonal [L2B10] to liver FABP
- Suitable for: Flow Cyt, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-liver FABP antibody [L2B10]
liver FABP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [L2B10] to liver FABP -
由来種
Mouse -
特異性
This monoclonal antibody binds to human liver FABP of both natural and recombinant origin. No reactivity to human intestinal or heart FABP. -
アプリケーション
適用あり: Flow Cyt, IHC-P, IHC-Frmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Rat, Human, African green monkey -
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituent: 0.2% BSA -
Concentration information loading... -
精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
L2B10 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab7366 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
|
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. |
ターゲット情報
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機能
Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. -
配列類似性
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
ドメイン
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 2168 Human
- Entrez Gene: 14080 Mouse
- Entrez Gene: 24360 Rat
- Omim: 134650 Human
- SwissProt: P07148 Human
- SwissProt: P12710 Mouse
- SwissProt: P02692 Rat
- Unigene: 380135 Human
see all -
別名
- FABP 1 antibody
- FABP1 antibody
- FABPL antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-liver FABP antibody [L2B10] (ab7366)
Immunohistochemical analysis of human L-FABP in frozen human kidney tissue with ab7366 at 1.9 μg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.co.jp/ps/products/7/ab7366/Images/ab7366-222193-anti-liver-fabp-antibody-l2b10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 200X.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.co.jp/ps/products/7/ab7366/Images/ab7366-222192-anti-liver-fabp-antibody-l2b10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-liver FABP antibody [L2B10] (ab7366)Immunohistochemistry (Paraffin-embedded sections) analysis of human kidney tissue labelling liver FABP with ab7366 at 1/1000. Antigen retrieval was performed using glycine buffer. Magnification: 50X.
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![Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)](https://www.abcam.co.jp/ps/products/7/ab7366/Images/ab7366-12927-anti-liver-fabp-antibody-l2b10-flow-cytometry.jpg)
Flow Cytometry - Anti-liver FABP antibody [L2B10] (ab7366)Overlay histogram showing HepG2 cells stained with ab110336 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab110336, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
プロトコール
参考文献 (12)
ab7366 は 12 報の論文で使用されています。
- Niture S et al. TNFAIP8 regulates autophagy, cell steatosis, and promotes hepatocellular carcinoma cell proliferation. Cell Death Dis 11:178 (2020). PubMed: 32152268
- Shen XY et al. Molecular classification of hepatocellular adenoma: A single-center experience. Ann Hepatobiliary Pancreat Surg 23:109-114 (2019). PubMed: 31225410
- Lyons J et al. The colonic epithelium plays an active role in promoting colitis by shaping the tissue cytokine profile. PLoS Biol 16:e2002417 (2018). PubMed: 29596476
- Takahashi T et al. Histopathological characteristics of glutamine synthetase-positive hepatic tumor lesions in a mouse model of spontaneous metabolic syndrome (TSOD mouse). Mol Clin Oncol 5:267-270 (2016). IHC-P ; Mouse . PubMed: 27446562
- Smathers RL et al. Susceptibility of L-FABP-/- mice to oxidative stress in early-stage alcoholic liver. J Lipid Res 54:1335-45 (2013). WB ; Mouse . PubMed: 23359610
