Liver Carboxylesterase 1 overexpression 293T lysate (whole cell) (ab94047)
製品の概要
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製品名
Liver Carboxylesterase 1 overexpression 293T lysate (whole cell)
Liver Carboxylesterase 1/CES1 ライゼート 製品一覧 -
特記事項
ab94047 is a 293T cell transfected lysate in which Human Liver Carboxylesterase 1 has been transiently over-expressed using a pCMV-Liver Carboxylesterase 1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究分野
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背景
Function: Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Catalyzes the transesterification of cocaine to form cocaethylene. Displays fatty acid ethyl ester synthase activity, catalyzing the ethyl esterification of oleic acid to ethyloleate. PTM: Contains sialic acid. Cleavage of the signal sequence can occur at 2 positions, either between Trp-17 and Gly-18 or between Gly-18 and His-19. Similarity: Belongs to the type-B carboxylesterase/lipase family. Tissue specificity: Expressed predominantly in liver with lower levels in heart and lung.
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94047の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution.
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特記事項 |
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WB
Use at an assay dependent dilution. |
画像
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ab94047 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-Liver Carboxylesterase 1/CES1 antibody (ab89779) at 1/500 dilution
Lane 1 :Liver Carboxylesterase 1 overexpression 293T lysate (whole cell) (ab94047)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab94047 は論文での使用が確認できていません。