Anti-Liver Arginase 抗体 [EPR6671(B)] - BSA and Azide free (ab248058)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6671(B)] to Liver Arginase - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Liver Arginase antibody [EPR6671(B)] - BSA and Azide free
Liver Arginase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6671(B)] to Liver Arginase - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HepG2 and A549 cell lysates. Human liver, Human fetal liver, and Mouse liver lysate tissue lysate.
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特記事項
ab248058 is the carrier-free version of ab124917.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6671(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248058の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa. |
ターゲット情報
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パスウェイ
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
関連疾患
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
配列類似性
Belongs to the arginase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 383 Human
- Entrez Gene: 11846 Mouse
- Entrez Gene: 29221 Rat
- Omim: 608313 Human
- SwissProt: P05089 Human
- SwissProt: Q61176 Mouse
- SwissProt: P07824 Rat
- Unigene: 440934 Human
see all -
別名
- A I antibody
- Al antibody
- ARG 1 antibody
see all
画像
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All lanes : Anti-Liver Arginase antibody [EPR6671(B)] (ab124917) at 1/1000 dilution
Lane 1 : Wild-type HepG2 cell lysate at 20 µg
Lane 2 : arg1 knockout HepG2 cell lysate at 20 µg
Lanes 3 & 5 : Empty at 0 µg
Lane 4 : Human Liver cell lysate at 5 µg
Lane 6 : A549 cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-Liver Arginase antibody [EPR6671(B)] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab124917 was shown to bind specifically to Liver Arginase. A band was observed at 36 kDa in wild-type HepG2 cell lysates with no signal observed at this size in arg1 knockout cell line ab281603 (knockout cell lysate ab282955). Faint band remaining in KO sample at same molecular weight is likely to be an isoform of arg1. To generate this image, wild-type and arg1 knockout HepG2 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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All lanes : Anti-Liver Arginase antibody [EPR6671(B)] (ab124917) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Mouse liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 35 kDaThis data was developed using ab124917, the same antibody clone in a different buffer formulation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248058 は論文での使用が確認できていません。