Anti-Liver Arginase 抗体 [ARG1/1125] (ab215843)
Key features and details
- Mouse monoclonal [ARG1/1125] to Liver Arginase
- Suitable for: Protein Array, IHC-P, WB
- Reacts with: Human
- Isotype: IgG3
製品の概要
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製品名
Anti-Liver Arginase antibody [ARG1/1125]
Liver Arginase 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [ARG1/1125] to Liver Arginase -
由来種
Mouse -
アプリケーション
適用あり: Protein Array, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment within Human Liver Arginase aa 1-150. The exact sequence is proprietary. 87 amino acids long. NCBI Accession No. 440934.
Database link: P05089 -
ポジティブ・コントロール
- Human hepatocellular carcinoma tissue. 293T cells. Huma liver lysate. Recombinant Human Liver Arginase protein fragment.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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精製度
Protein A/G purified -
ポリ/モノ
モノクローナル -
クローン名
ARG1/1125 -
アイソタイプ
IgG3 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab215843の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Protein Array |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 2 - 4 µg/ml.
Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.
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特記事項 |
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Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 2 - 4 µg/ml. Primary incubation for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa. |
ターゲット情報
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パスウェイ
Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1. -
関連疾患
Defects in ARG1 are the cause of argininemia (ARGIN) [MIM:207800]; also known as hyperargininemia. Argininemia is a rare autosomal recessive disorder of the urea cycle. Arginine is elevated in the blood and cerebrospinal fluid, and periodic hyperammonemia occurs. Clinical manifestations include developmental delay, seizures, mental retardation, hypotonia, ataxia, progressive spastic quadriplegia. -
配列類似性
Belongs to the arginase family. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 383 Human
- Omim: 608313 Human
- SwissProt: P05089 Human
- Unigene: 440934 Human
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別名
- A I antibody
- Al antibody
- ARG 1 antibody
see all
画像
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All lanes : Anti-Liver Arginase antibody [ARG1/1125] (ab215843) at 1 µg/ml
Lane 1 : recombinant Human Liver Arginase protein fragment
Lane 2 : Human liver lysate
Predicted band size: 35 kDa -
Immunohistochemical analysis of formalix-fixed paraffin-embedded Human hepatocellular carcinoma tissue, labeling Liver Arginase using ab215843 at 4 µg/mL.
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ab215843 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab215843 は論文での使用が確認できていません。