Anti-Listeria monocytogenes 抗体 (ab20506)

Submit a review Q&A (4)References (1)

Key features and details

  • Rabbit polyclonal to Listeria monocytogenes
  • Suitable for: ELISA
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Listeria monocytogenes antibody
    Listeria monocytogenes 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Listeria monocytogenes

  • 由来種

    Rabbit

  • 特異性

    Recognizes whole cells of Listeria monocytogenes.

  • アプリケーション

    適用あり: ELISAmore details

  • 種交差性

    交差種: Other species

  • 免疫原

    Tissue, cells or virus corresponding to Listeria monocytogenes. Listeria monocytogenes, ATCC 43251

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab20506の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
特記事項
ELISA
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    The genus Listeria comprises six species: L. monocytogenes, L. innocua, L. welshimeri, L. seeligeri, L. ivanovii and L. grayi. Listeria monocytogenes, the most commonly isolated pathogenic member, is associated with a wide spectrum of human and animal diseases. In the smear from the original tissue, L. monocytogenes may appear as gram-positive coccobacilli that may be confused with Streptococcus agalactiae (group B), enterococci, or Corynebacterium spp. Listeria is differentiated from streptococci by a positive catalase test. L. monocytogenes is the only species of the genus Listeria that has been clearly documented as a pathogen for humans. The forms of disease caused by this organism are myriad and age-related. The most common clinical manifestations are meningitis and septicemia. Listeria monocytogenes, a food-borne intracellular animal and human pathogen, interacts with infected host cells both prior to entry and during the intracellular phase of infection.

  • 別名

    • L. monocytogenes antibody

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab20506 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab20506 は 1 報の論文で使用されています。

  • Xu Y  et al. A Bacterial Effector Reveals the V-ATPase-ATG16L1 Axis that Initiates Xenophagy. Cell 178:552-566.e20 (2019). PubMed: 31327526

レビューと Q&A

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1-4 of 4 Abreviews or Q&A

Question

Dear abcam team
I am interested in:
Anti-Listeria monocytogenes H-AB antibody (ab79487)
Could this product be used to coat magnetic beads?
(Which subsequently could be used to separate flagellated cells).
What's the actual price for this product?

Thank you very much for your answer in advance.
Sincerely,

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Answer

Thank you for your enquiry and your interest.

I can confirm that this antibody has only been tested for Agglutination - but not for IP (immunoprecipitaion) or conjugation. Alternatively, you may be interested in ab20506 which has been characterized for conjugation and ELISA. The price and unit size can be viewed on the on-line product datasheet once you select the home country.

The shipping, handling & packaging charges can be found on our website as well:

https://www.abcam.com/index.html?pageconfig=help#shippingcharges

I hope this helps and if I can assist further, please do not hesitate to contact me.

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Question

Dear All, our customer is studying Listeria monocytogenes and she is interested in you antibodies but she would like to use them in live cells. Do your antibodies (here below) work in live cells?  

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Answer

Thank you for your enquiry. To help answer your customer's question, could you please let me know what specific application they will use the antibodies for (eg. western blotting, IHC, etc). Thanks!

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Question

I saw that AB20506 is tested for “conjugation”. What do you mean exactly for “conjugation”,? With fluorocrome and/or enzyme? Our customer would like to conjugate AB20506 to magnetic beads. Do you think this Ab is suitable for that? If so, do you have any protocol to suggest?

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Answer

Thank you for contacting us. The Listeria monocytogenes antibody ab20506 has been validated for conjugation to biotin, FITC, and HRP. We have not conjugated it to magnetic beads in house, but it should be suitable for that purpose. Our protocol for coupling antibodies to Protein A or G beads can be found on the following site: https://www.abcam.com/index.html?pageconfig=resource&rid=11415 For magnetic beads, I would recommend following the protocol provided by the manufacturer of the beads. I hope this helps, please let me know if you need any additional information.

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Question

Dear sir/madame, I am a PhD candidate from the Chemical department in the University oF Ioanniana, Greece. We are interested about the Listeria monocytogenes antibody with code ab20506 and i need some information about it. Did this antibody recognize heat-killed Listeria monocytogenes cells? Which immunogen had been used for its preparation? For the ab20766 you mention the ATCC 43251....... Best regards

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Answer

Thank you for your enquiry. The immunogen used for Rabbit anti Listeria monocytogenes ab20506 is a mixture of intact and disrupted L. monocytogenes organisms. We have not tested this antibody for reactivity with heat-killed L. monocytogenes cells. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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