Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1.5µg/mL · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citra Plus
Sample
Mouse Tissue sections (Small Intestine)
Specification
Small Intestine
Permeabilization
No
Fixative
Formaldehyde
Other product details
Concentration
2 µg/ml
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Victrastain Dillution buffer
Secondary antibody
Name
Non-Abcam antibody was used: VictraStain Anti-Mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 28 2013