製品の概要

  • 製品名

    Lipolysis Assay Kit (Fluorometric)
    Lipolysis キット 製品一覧
  • 検出方法

    Fluorescent
  • サンプルの種類

    Cell culture supernatant
  • アッセイタイプ

    Quantitative
  • 検出感度

    < 0.02 nmol/well
  • 種交差性

    交差種: Other species, Mammals
  • 製品の概要

    Lipolysis Assay Kit (Fluorometric) ab185434 is simple, easy-to-use and highly sensitive.


    This lipolysis assay measures glycerol released from 3T3-L1 cells as early as 1 hour after induction of lipolysis. It is suitable for measuring trace amounts of glycerol from samples. In the assay, glycerol reacts with Glycerol Enzyme Mix to form an intermediate that in turn reacts with Glycerol Developer and PicoProbe™ to generate the fluorescence product. The fluorescence intensity is directly proportional to the amount of glycerol.


    The lipolysis assay kit can detect less than 20 pmol of glycerol.

  • 特記事項

    Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue.

  • 試験プラットフォーム

    Microplate reader

製品の特性

  • 保存方法

    Store at -20°C. Please refer to protocols.
  • 内容 ラベル 100 tests
    Glycerol Assay Buffer WM 1 x 25ml
    Glycerol Developer 1 vial
    Glycerol Enzyme Mix Green 1 vial
    Glycerol Standard (100 mM) Yellow 1 x 0.2ml
    Isoproterenol (10mM) 1 x 50µl
    Lipolysis Assay Buffer 1 x 17ml
    Lipolysis Wash Buffer 1 x 22ml
    PicoProbe Blue 1 x 0.4ml
  • 研究分野

画像

  • 5 μl of 3T3-L1 cells treated with vehicle control (H2O) or 100 nM Isoproterenol for 0-3 hours. Measurements for 0 hours were undetectable.

  • Inhibition of Isoproterenol (100 nM) stimulated lipolysis by treatment with 100 nM insulin (measured using 5 μl of media of 3T3-L1 cells). Control was vehicle (H2O) treated cells.

プロトコール

参考文献

ab185434 has not yet been referenced specifically in any publications.

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