Application
Western blot
Sample
Human Cell lysate - whole cell (human sputum)
Loading amount
20 µg
Specification
human sputum
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
17 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: mouse anti rabbit IgG
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Human
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 21 kDa
Positive control
positive control os neutrophil cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Taehyeong lee
Verified customer
投稿 Nov 16 2012