Application
Western blot
Sample
Rat Cell lysate - whole cell (Hepatocytes)
Loading amount
50 µg
Specification
Hepatocytes
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Skim Milk in PBS-T20 Buffer
Secondary antibody
Name
Non-Abcam antibody was used: Swine anti-rabbit immunoglobulin
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: approximately 23 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Shakil Ahmad
Verified customer
投稿 Aug 09 2011