Application
Western blot
Sample
Human Cell lysate - whole cell (Neutrophils)
Gel Running Conditions
Reduced Denaturing (12% GEL)
Loading amount
25 µg
Specification
Neutrophils
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Cell Signaling - Affinity purified horse anti-mous
Host species: Horse
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Horse
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL+
Exposure
5 second(s)
Bands
Specific: 25 kDa
Positive control
human neutrophils
Additional data
Additional Notes
Each lane are corresponding to three different human samples with same pathology (severe infection).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MME. Louise Chomel
Verified customer
投稿 Jul 30 2021