Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970)
製品の概要
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製品名Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric)
Lipid Peroxidation キット 製品一覧 -
検出方法Colorimetric/Fluorometric
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サンプルの種類Plasma, Cell culture extracts, Tissue Extracts
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アッセイタイプQuantitative
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検出感度> 0.1 nmol/well
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全工程の試験時間1h 20m
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製品の概要
Lipid Peroxidation (MDA) Assay Kit (Colorimetric/Fluorometric) (ab118970) provides a convenient tool for sensitive detection of malondialdehyde (MDA). MDA, together with 4-hydroxynonenal (4-HNE), is a natural bi-product of lipid peroxidation and its quantification is generally used as a marker for lipid peroxidation.
In the lipid peroxidation assay protocol, the MDA in the sample reacts with thiobarbituric acid (TBA) to generate a MDA-TBA adduct. The MDA-TBA adduct can be easily quantified colorimetrically (OD = 532 nm) or fluorometrically (Ex/Em = 532/553 nm). This assay detects MDA levels as low as 1 nmol/well colorimetrically and 0.1 nmol/well fluorometrically.
The MDA assay is also refered to as a TBARS assay.
Lipid peroxidation assay protocol summary:
- add TBA solution to samples and standards, incubate at 95ºC for 60 min, cool in ice bath for 10 min
- transfer to wells of microplate
- analyze with microplate reader
For higher sensitivity, precipitate with n-butanol, centrifuge, dry and resuspend pellet before analysis.
For an alternative MDA assay, without the heating steps required in the TBARS assay, try MDA assay ab233471.
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特記事項
Lipid peroxidation refers to the oxidative degradation of lipids. In this process free radicals take electrons from the lipids (generally in cell membranes), resulting in cell damage. Quantification of lipid peroxidation is essential to assess oxidative stress. Lipid peroxidation forms reactive aldehydes such as malondialdehyde (MDA) and 4-hydroxynonenal (4- HNE) as natural bi-products. Measuring the end products of lipid peroxidation is one of the most widely accepted assays for oxidative damage.
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Review the oxidative stress marker and assay guide, or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader.
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試験プラットフォームMicroplate reader
製品の特性
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保存方法Store at -20°C. Please refer to protocols.
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内容 ラベル 100 tests BHT (100X) Purple 1 x 1ml MDA Lysis Buffer WM 1 x 25ml MDA Standard (4.17M) Yellow 1 x 100µl Phosphotungstic Acid Solution NM 1 x 12.5ml TBA NM 4 vials -
研究分野
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関連性Lipid peroxidation refers to the oxidative degradation of lipids and is a well-defined mechanism of cellular damage. The formation of lipid peroxidation products leads to spread of free radical reactions leading to cell damage.
関連製品
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画像
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Lipid peroxidation (malondialdehyde [MDA]) levels were measured in cavernosal tissue using ab118970Park J et al., PLoS One, 12(2). Fig 4a. doi: 10.1371/journal.pone.0172751
10 mg of tissue were homogenized on ice in 300 μL of MDA lysis buffer, then centrifuged (13,000 × g, 10 min) to remove insoluble materials. 10 μL of plasma were mixed with 500 μL of 42 mM H2SO4 and 125 μL of phosphotungstic acid solution at room temperature for 5 min. After centrifuging (13,000 × g, 3 min), the pellet was re-suspended on ice with 100 μL of double-distilled H2O. Then, 200 μL of solution and 600 μL of 2-thiobarbituric acid solution were incubated at 95°C for 60 min, before cooling to room temperature in the ice bath for 10 min. The intensity of absorbance at 532 nm was proportional to the MDA level.
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Typical MDA standard calibration curve using colorimetric reading.
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Typical MDA standard calibration curve using fluorometric reading.
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Measurement of MDA in human plasma (20 µl) and rat liver lysate (10 mg).
プロトコール
参考文献
This product has been referenced in:
- Hichor M et al. Liver X Receptor exerts a protective effect against the oxidative stress in the peripheral nerve. Sci Rep 8:2524 (2018). Read more (PubMed: 29410501) »
- Hai B et al. Delivery of Sonic Hedgehog Gene Repressed Irradiation-induced Cellular Senescence in Salivary Glands by Promoting DNA Repair and Reducing Oxidative Stress. Theranostics 8:1159-1167 (2018). Read more (PubMed: 29464006) »