Lipid Peroxidation (MDA) Assay Kit (Colorimetric) (ab233471)
Key features and details
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells, Tissue Lysate
製品の概要
-
製品名
Lipid Peroxidation (MDA) Assay Kit (Colorimetric)
Lipid Peroxidation キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Adherent cells, Suspension cells, Tissue Lysate -
製品の概要
Lipid Peroxidation (MDA) Assay Kit (Colorimetric) ab233471 enables researchers to detect MDA without the heating steps required by the TBARS assay conventionally used for MDA detection.
In this MDA assay, the MDA Color Reagent reacts with MDA to generate a blue color product which is measured at 695 nm with absorbance microplate readers. The assay is very fast and specific for MDA with little interference from other aldehydes.
Alternatively, see our popular TBARS assay kit for MDA measurement ab118970.
MDA assay protocol summary for ab233471:
- add samples and standards to wells
- add MDA color reagent and incubate for 10-30 min at room temp
- add reaction solution and incubate for 30-60 min at room temp
- analyze with microplate reader -
特記事項
Lipid peroxidation is characterized by the oxidative degradation of unsaturated fatty acids, phospholipids, glycolipids, cholesterol esters and cholesterol. Malondialdehyde (MDA) is one of the most commonly used biomarkers for lipid peroxidation.
Running an MDA assay has historically relied on a reaction with thiobarbituric acid (the TBARS assay) to generate a product that can be measured colorimetrically at 532 nm or fluorimetrically at Ex/Em = 530/550 nm.
However, the TBARS assay has quite a few limitations:
- the reaction is not specific to MDA,
- the TBA-MDA reaction needs be run under acidic conditions,
- the TBARS assay needs be run under high temperature, commonly at 90-100 ºC. -
アプリケーション
適用あり: Functional Studiesmore details -
試験プラットフォーム
Microplate reader
製品の特性
-
保存方法
Store at -20°C. Please refer to protocols. -
内容 200 tests Dilution Buffer 1 x 10ml MDA Color Reagent 1 vial MDA Standard 1 vial Reaction Solution 1 x 10ml
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233471の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Functional Studies |
Use at an assay dependent concentration.
|
特記事項 |
---|
Functional Studies
Use at an assay dependent concentration. |
画像
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (16)
ab233471 は 16 報の論文で使用されています。
- Wang X et al. Ferroptosis is essential for diabetic cardiomyopathy and is prevented by sulforaphane via AMPK/NRF2 pathways. Acta Pharm Sin B 12:708-722 (2022). PubMed: 35256941
- Gad El-Hak HN et al. Methanolic Phoenix dactylifera L. Extract Ameliorates Cisplatin-Induced Hepatic Injury in Male Rats. Nutrients 14:N/A (2022). PubMed: 35268000
- Yang Y et al. The Activation of AMPK/NRF2 Pathway in Lung Epithelial Cells Is Involved in the Protective Effects of Kinsenoside on Lipopolysaccharide-Induced Acute Lung Injury. Oxid Med Cell Longev 2022:3589277 (2022). PubMed: 35340214
- Wen C et al. GSK3ß Exacerbates Myocardial Ischemia/Reperfusion Injury by Inhibiting Myc. Oxid Med Cell Longev 2022:2588891 (2022). PubMed: 35528516
- Zhu X et al. Neuroprotective Effect of E3 Ubiquitin Ligase RNF8 Against Ischemic Stroke via HDAC2 Stability Reduction and Reelin-Dependent GSK3ß Inhibition. Mol Neurobiol 59:4776-4790 (2022). PubMed: 35622272