Anti-Lipid A 抗体 [26-5] (ab8467)
Key features and details
- Mouse monoclonal [26-5] to Lipid A
- Suitable for: Agglutination, ELISA
- Reacts with: Species independent
- Isotype: IgG2b
製品の概要
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製品名
Anti-Lipid A antibody [26-5] -
製品の詳細
Mouse monoclonal [26-5] to Lipid A -
由来種
Mouse -
アプリケーション
適用あり: Agglutination, ELISAmore details -
種交差性
交差種: Species independent -
免疫原
Other Immunogen Type corresponding to Lipid A. Synthetic Lipid A.
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特記事項
Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 99.88% PBS -
Concentration information loading...
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精製度
IgG fraction -
特記事項(精製)
This product is sterile filtered. -
一次抗体 備考
Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria. -
ポリ/モノ
モノクローナル -
クローン名
26-5 -
ミエローマ
unknown -
アイソタイプ
IgG2b -
軽鎖の種類
unknown -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8467の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Agglutination |
Use at an assay dependent concentration.
Use NEAT. |
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ELISA |
1/10.
in phosphate buffered saline containing 4% BSA and 0.05% Tween.For ELISA prepare bacteria suspension in PBS (5x10^8 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density. |
特記事項 |
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Agglutination
Use at an assay dependent concentration. Use NEAT. |
ELISA
1/10. in phosphate buffered saline containing 4% BSA and 0.05% Tween.For ELISA prepare bacteria suspension in PBS (5x10^8 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density. |
ターゲット情報
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関連性
Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction. -
細胞内局在
Secreted
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab8467 は 5 報の論文で使用されています。
- Kameli N et al. Characterization of Feces-Derived Bacterial Membrane Vesicles and the Impact of Their Origin on the Inflammatory Response. Front Cell Infect Microbiol 11:667987 (2021). PubMed: 34026664
- Schlichtner S et al. Functional role of galectin-9 in directing human innate immune reactions to Gram-negative bacteria and T cell apoptosis. Int Immunopharmacol 100:108155 (2021). PubMed: 34543981
- Vargas-Caraveo A et al. Lipopolysaccharide enters the rat brain by a lipoprotein-mediated transport mechanism in physiological conditions. Sci Rep 7:13113 (2017). PubMed: 29030613
- Martin I et al. Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo. J Immunol 194:3924-36 (2015). PubMed: 25780044
- Kadowaki T et al. Functional Characterization of Lipopolysaccharide derived from Symbiotic Bacteria in Rice as a Macrophage-activating Substance. Anticancer Res 31:2467-76 (2011). WB . PubMed: 21873161