Key features and details
- Mouse monoclonal [26-5] to Lipid A
- Suitable for: Agglutination, ELISA
- Reacts with: Species independent
- Isotype: IgG2b
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
バッファーPreservative: 0.02% Sodium azide
Constituent: 0.1% BSA
Concentration information loading...
特記事項（精製）This product is sterile filtered.
一次抗体 備考Lipid A is part of LPS (lipopolysaccaride) present in gram-negative bacteria.
Our Abpromise guarantee covers the use of ab8467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Agglutination||Use at an assay dependent concentration. Use NEAT.|
in phosphate buffered saline containing 4% BSA and 0.05% Tween.For ELISA prepare bacteria suspension in PBS (5x10^8 bact./ml). Coat ELISA plate with 100 µl/well for 1 h. at 37°C, store overnight at 4°C and wash 5x with tapwater with 0.05% Tween (TT). Incubate with diluted antibody, 1 h. at 37°C. Wash 5x with TT. Incubate with appropriate conjugate (anti-mouse Ig enzyme labeled antibody). Add substrate solution, incubate, stop reaction and read optical density.
関連性Lipid A is a lipid component of an endotoxin held responsible for toxicity of Gram-negative bacteria. Sensing of lipid A by the human immune system may also be critical for the onset of immune responses to Gram-negative infection, and for the subsequent successful fight against the infection. Lipid A is located at one end of the lipopolysaccharide (LPS, also called endotoxin) molecule, and anchors the LPS to the outer membrane of a Gram-negative bacteria. Many of the immune activating abilities of LPS can be contributed to the lipid A unit. It is a very potent stimulant of the immune system, activating cells (for example, monocytes or macrophages) at picogram per milliliter quantities. When present in the body at high concentrations during a Gram-negative bacterial infection, it may cause shock and death by an "out of control" excessive immune reaction.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab8467 は 3 報の論文で使用されています。
- Vargas-Caraveo A et al. Lipopolysaccharide enters the rat brain by a lipoprotein-mediated transport mechanism in physiological conditions. Sci Rep 7:13113 (2017). PubMed: 29030613
- Martin I et al. Fasciola hepatica fatty acid binding protein inhibits TLR4 activation and suppresses the inflammatory cytokines induced by lipopolysaccharide in vitro and in vivo. J Immunol 194:3924-36 (2015). PubMed: 25780044
- Kadowaki T et al. Functional Characterization of Lipopolysaccharide derived from Symbiotic Bacteria in Rice as a Macrophage-activating Substance. Anticancer Res 31:2467-76 (2011). WB . PubMed: 21873161