Lipase Assay Kit III (Fluorometric) (ab118969)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell culture supernatant, Milk, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.0001 mU/well
製品の概要
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製品名
Lipase Assay Kit III (Fluorometric)
Lipase キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media -
アッセイタイプ
Enzyme activity -
検出感度
> 0.0001 mU/well -
全工程の試験時間
1h 00m -
種交差性
交差種: Mammals, Other species -
製品の概要
Lipase Activity Assay Kit III (Fluorometric) (ab118969) is a lipase activity assay where lipase hydrolyzes a specific substrate to generate the methylresorufin, which can be detected fluorometrically at Ex/Em=529/600 nm. This kit can be used to detect Lipase as low as 0.1 µU/well.
The lipase assay kit provides a rapid, simple, sensitive, and reliable test suitable for high throughput assay of Lipase activity.
Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyse with a microplate reader immediately and at 30-60 min -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K724 Lipase Activity Fluorometric Assay Kit III. K724-100 is the same size as the 100 test size of ab118969.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer V 1 x 25ml Lipase Positive Control 1 vial Lipase Substrate I 1 x 200µl Methylresorufin Standard 1 x 40µl -
研究分野
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関連性
Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours.
関連製品
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab118969 は 2 報の論文で使用されています。
- Humpton TJ et al. Differential requirements for MDM2 E3 activity during embryogenesis and in adult mice. Genes Dev 35:117-132 (2021). PubMed: 33334825
- Huang L et al. Commitment and oncogene-induced plasticity of human stem cell-derived pancreatic acinar and ductal organoids. Cell Stem Cell 28:1090-1104.e6 (2021). PubMed: 33915081