Lipase Assay Kit II (Colorimetric) (ab102525)

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製品の概要

  • 製品名

    Lipase Assay Kit II (Colorimetric)
    Lipase キット 製品一覧

  • 検出方法

    Colorimetric

  • サンプルの種類

    Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts

  • アッセイタイプ

    Enzyme activity

  • 全工程の試験時間

    1h 30m

  • 製品の概要

    Lipase Assay Kit II (Colorimetric) (ab102525) provides a simple, sensitive, and reliable assay for rapid analysis of Lipase activity.


    In the lipase assay, Lipases hydrolyze a specific substrate to generate a product which reacts with the DTNB probe to generate color (Absorbance=412 nm). This kit is suitable for high throughput analysis (HTP).


    Lipase assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze with a microplate reader at 3 min and at between 60 and 90 min

  • 試験プラットフォーム

    Microplate reader

製品の特性

画像

  • Functional Studies - Lipase Detection Kit II (ab102525)
    Functional Studies - Lipase Detection Kit II (ab102525)
    Lipase Detection in a Sample and Positive Control
  • Functional Studies - Lipase Detection Kit II (ab102525)
    Functional Studies - Lipase Detection Kit II (ab102525)
    TNB Standard Curve

プロトコール

参考文献

ab102525 has not yet been referenced specifically in any publications.

ab102525 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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Question

Hello,

I have a question regarding your Lipase Detection Kit II (product code ab102525) and Lipase Detection Kit III (ab118969). In order to determine if these products might be potentially useful for our application, I would need to know more about the structures of the substrates.

I understand that you can probably not release full detailed information regarding the chemical structure. I would just like to know if the substrates are triglyceride derivatives, or if they are more simple esters with only one hydrophobic fatty acid like chain.

I look forward to hearing from you.

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Answer

Thank you for contacting Abcam.

The substates for ab102525 and ab118969 are triglyceride derivatives.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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