Lipase Assay Kit (Colorimetric) (ab102524)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell culture media, Cell culture supernatant, Milk, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.02 mU/well
製品の概要
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製品名
Lipase Assay Kit (Colorimetric)
Lipase キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Milk, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell culture media -
アッセイタイプ
Enzyme activity -
検出感度
0.02 mU/well -
全工程の試験時間
1h 30m -
製品の概要
Lipase Assay Kit ab102524 is a rapid, simple, and sensitive colorimetric assay for the measurement of lipase activity.
In the lipase assay protocol, lipase hydrolyzes a triglyceride substrate to form glycerol which is quantified enzymatically by monitoring a linked change in the absorbance of a probe (OD=570nm). This kit is suitable for high throughput analysis (HTP).
This lipase assay kit detects lipase activity as low as 0.02mU per well.
Lipase assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze every 2-3 min with microplate reader in kinetic mode for at least 60-90 min at 37ºC -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K722 Lipase Activity Colorimetric Assay Kit. K722-100 is the same size as the 100 test size of ab102524.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Assay Buffer V 1 x 25ml Enzyme Mix VI 1 vial Glycerol Standard 1 x 0.2ml Lipase Positive Control 1 vial Lipase Substrate III 1 vial OxiRed Probe 1 x 0.2ml -
研究分野
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関連性
Lipases perform essential roles in the digestion, transport and processing of dietary lipids (e.g. fats and oils) in living organisms. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglyceride to monoglyceride and free fatty acid. Pancreatic lipase monitoring is also used to help diagnose Crohn's disease, cystic fibrosis and celiac disease. Damage to the pancreas can exhibit a 5-10 fold increase of serum lipase levels within 24 to 48 hours.
関連製品
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Related Products
画像
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Plasma lipase levels were measured (using ab102524) after 75 days treatment with saline, liraglutide, exendin-4 or sitagliptin. ND, normal chow diet; HFD, high fat diet. p≤0.05, *; p≤0.01, **, n = 3–7 mice.
There were no detectable differences in plasma lipase activity in mice on a normal chow diet administered any of the three drugs when compared to animals administered saline. Likewise, there was no significant change in plasma lipase activity in mice that were administered saline on a high fat diet vsnormal diet. Furthermore, administration of liraglutide and exendin-4 in combination with a high fat diet also failed to affect plasma lipase activity. We observed no detectable changes in plasma lipase activity in animals maintained on a normal chow diet and administered any of the three drugs when compared to animals administered saline.
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Sample Timeline
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Glycerol standard Curve
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab102524 は 21 報の論文で使用されています。
- Bartolacci C et al. Targeting de novo lipogenesis and the Lands cycle induces ferroptosis in KRAS-mutant lung cancer. Nat Commun 13:4327 (2022). PubMed: 35882862
- Tung YT et al. Ascophyllum nodosum and Fucus vesiculosus Extracts Improved Lipid Metabolism and Inflammation in High-Energy Diet-Induced Hyperlipidemia Rats. Nutrients 14:N/A (2022). PubMed: 36364926
- Arévalo Sureda E et al. Exocrine Pancreatic Maturation in Pre-term and Term Piglets Supplemented With Bovine Colostrum. Front Nutr 8:687056 (2021). PubMed: 34249996
- Dolai S et al. Pancreas-specific SNAP23 depletion prevents pancreatitis by attenuating pathological basolateral exocytosis and formation of trypsin-activating autolysosomes. Autophagy 17:3068-3081 (2021). PubMed: 33213278
- Wu J et al. Treatment of Severe Acute Pancreatitis and Related Lung Injury by Targeting Gasdermin D-Mediated Pyroptosis. Front Cell Dev Biol 9:780142 (2021). PubMed: 34858995