Anti-LINE-1 ORF2p 抗体 (ab106004)
Key features and details
- Chicken polyclonal to LINE-1 ORF2p
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgY
製品の概要
-
製品名
Anti-LINE-1 ORF2p antibody
LINE-1 ORF2p 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to LINE-1 ORF2p -
由来種
Chicken -
特異性
The two synthetic immunogen peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein. This antibody is intended for use in studying control of L1 retrotransposons.
-
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee -
免疫原
Synthetic peptide corresponding to Human LINE-1 ORF2p conjugated to keyhole limpet haemocyanin. Two synthetic peptides conjugated to keyhole limpet hemocyanin (KLH). The peptides correspond to regions within the endonuclease domain of LINE-1 ORF2p protein.
Database link: O00370 -
ポジティブ・コントロール
- induced bacterially expressed human ORF2
-
特記事項
Dilute only prior to immediate use.
Expiration date is one (1) year from date of opening product.ab106004 is intended for use in studying control of L1 retrotransposons.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading...
-
精製度
IgY fraction -
特記事項(精製)
ab106004 is an IgY fraction antibody purified from monospecific Chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation, followed by extensive dialysis against the buffer. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106004の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
WB |
1/2000 - 1/10000. Predicted molecular weight: 149 kDa.
|
特記事項 |
---|
ELISA
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Predicted molecular weight: 149 kDa. |
ターゲット情報
-
参照データベース
- SwissProt: O00370 Human
-
別名
- L1/ORF2 antibody
- LINE 1 ORF2p antibody
- LINE-1 retrotransposable element ORF2 protein antibody
see all
画像
-
All lanes : Anti-LINE-1 ORF2p antibody (ab106004) at 1/2000 dilution
Lane 1 : induced bacterially expressed human ORF2
Lane 2 : uninduced bacterially expressed human ORF2
Predicted band size: 149 kDaNote: No specific band staining is seen in the uninduced lane (lane 2). The lower molecular weight bands represent non-specific staining. The band at ~70 kDa (arrowhead) corresponds to a Human LINE-1 ORF2p endonuclease domain fusion protein.
データシートおよび資料
-
Datasheet download
参考文献 (4)
ab106004 は 4 報の論文で使用されています。
- Zhang H et al. Single-nucleus transcriptomic landscape of primate hippocampal aging. Protein Cell 12:695-716 (2021). PubMed: 34052996
- Bi S et al. SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer. Protein Cell 11:483-504 (2020). PubMed: 32504224
- Romero MA et al. Acute and chronic resistance training downregulates select LINE-1 retrotransposon activity markers in human skeletal muscle. Am J Physiol Cell Physiol 314:C379-C388 (2018). PubMed: 29351416
- Thomas CA et al. Modeling of TREX1-Dependent Autoimmune Disease using Human Stem Cells Highlights L1 Accumulation as a Source of Neuroinflammation. Cell Stem Cell 21:319-331.e8 (2017). PubMed: 28803918