Key features and details
- Rabbit polyclonal to LIN7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の詳細Rabbit polyclonal to LIN7
アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
交差が予測される動物種: Mouse, Rat, Cow, Zebrafish
Recombinant fragment, corresponding to a region within amino acids 1-187 of Human LIN7 (UniProt O14910).
- NT2D1, IMR32, U87-MG and MCF7 whole cell lysate, MCF7 cells, Human colon carcinoma tissue
特記事項Keep as a concentrated solution.
保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.025% Proclin 300
Constituents: PBS, 1% BSA, 20% Glycerol
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab127049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 22 kDa.|
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
機能Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells.
配列類似性Belongs to the lin-7 family.
Contains 1 L27 domain.
Contains 1 PDZ (DHR) domain.
ドメインThe kinase interacting site is required for proper delivery of ERBB2 to the basolateral membrane.
The PDZ domain regulates endocytosis and recycling of the receptor at the membrane.
The L27 domain mediates interaction with CASK and is involved in the formation of multimeric complexes and the association of LIN7 to membranes.
細胞内局在Cell membrane. Basolateral cell membrane. Cell junction. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell junction > tight junction. Cell junction > synapse > synaptosome. Enriched in synaptosomes and at epithelial cell-cell junctions (By similarity). Mainly basolateral in renal epithelial cells.
- Information by UniProt
- hLin 7 antibody
- hLin7 antibody
- LIN 7 antibody
Anti-LIN7 antibody (ab127049) at 1/5000 dilution + MCF7 whole cell
lysate at 30 µg
Predicted band size: 22 kDa
12% SDS PAGE
ab127049, at a dilution of 1/500, staining LIN7 in methanol-fixed MCF7 cells by Immunofluorescence. Lower image has been costained with Hoechst 33342.
ab127049, at a dilution of 1/250, staining LIN7 in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.