Anti-LILRB3 抗体 [FRAS92B] - BSA and Azide free (ab271297)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [FRAS92B] to LILRB3 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-LILRB3 antibody [FRAS92B] - BSA and Azide free
LILRB3 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [FRAS92B] to LILRB3 - BSA and Azide free -
由来種
Rat -
アプリケーション
適用あり: IHC-P, ICC/IF, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human non-hodgkin's lymphoma and tonsil tissue. ICC/IF: 293T cells. Flow Cyt (intra): 293T cells.
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特記事項
ab271297 is the carrier-free version of ab271287.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
FRAS92B -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271297の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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関連性
LILRB3 is a 631 amino acid type I transmembrane glycoprotein, which contains four immunoreceptor tyrosine-based inhibition motif (ITIM) sequences within a long cytoplasmic tail. Phosphorylation of the tyrosine residues within ITIMs is known to enable the binding and activation of protein tyrosine phosphatases, which act as cell signalling modulators and inhibitors of cell activation. LILRB3 may act as receptor for class I MHC antigens. -
細胞内局在
Membrane; Single-pass type I membrane protein. -
参照データベース
- Entrez Gene: 11025 Human
- Omim: 604820 Human
- SwissProt: O75022 Human
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別名
- CD85 antigen like family member A antibody
- CD85a antibody
- CD85a antigen antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded human non-hodgkin's lymphoma tissue labeling LILRB3 with ab271287 at 1.762µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human non-hodgkin's lymphoma. The section was incubated with ab271287 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271287).
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LILRB3 with ab271287 at 1.762µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human tonsil. The section was incubated with ab271287 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271287).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T cells labelling LILRB3 with ab271287 at 3.524µg/ml , followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
Confocal image showing membranous and cytoplasmic staining on 293T cells transfected with LILRB3-myc plasmid.
Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain Myc at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271287).
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with myc tagged LILRB3 construct cells labelling LILRB3 with ab271287 at0.881µg/ml (Right panel) compared with a ratmonoclonal IgG isotype control (Left panel). Goat F (ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab271287).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab271297 は論文での使用が確認できていません。