Anti-LILRB3 抗体 [FRAS92B] (ab271287)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] (ab271287)](https://www.abcam.co.jp/ps/products/271/ab271287/Images/ab271287-1-anticd85a-antibody-fras92b-immunohistochemistry-human-nonhodgkins-lymphoma.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] (ab271287)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [FRAS92B] to LILRB3
- Suitable for: Flow Cyt, IHC-P, ICC
- Reacts with: Human
製品の概要
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製品名
Anti-LILRB3 antibody [FRAS92B]
LILRB3 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [FRAS92B] to LILRB3 -
由来種
Rat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC HumanIHC-P Human
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免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- IHC-P: Human non-hodgkin's lymphoma and tonsil tissue. ICC: 293T cells. Flow Cyt: 293T cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
FRAS92B -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab271287 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC |
Human
|
| IHC-P |
Human
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt |
Use a concentration of 0.881 µg/ml.
|
|
| IHC-P | (1) |
Use a concentration of 1.762 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| ICC |
Use a concentration of 3.524 µg/ml.
|
| 特記事項 |
|---|
|
Flow Cyt
Use a concentration of 0.881 µg/ml. |
|
IHC-P
Use a concentration of 1.762 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
ICC
Use a concentration of 3.524 µg/ml. |
ターゲット情報
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関連性
LILRB3 is a 631 amino acid type I transmembrane glycoprotein, which contains four immunoreceptor tyrosine-based inhibition motif (ITIM) sequences within a long cytoplasmic tail. Phosphorylation of the tyrosine residues within ITIMs is known to enable the binding and activation of protein tyrosine phosphatases, which act as cell signalling modulators and inhibitors of cell activation. LILRB3 may act as receptor for class I MHC antigens. -
細胞内局在
Membrane; Single-pass type I membrane protein. -
参照データベース
- Entrez Gene: 11025 Human
- Omim: 604820 Human
- SwissProt: O75022 Human
- Unigene: 631592 Human
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別名
- CD85 antigen like family member A antibody
- CD85a antibody
- CD85a antigen antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] (ab271287)
Immunohistochemical analysis of paraffin-embedded human non-hodgkin's lymphoma tissue labeling LILRB3 with ab271287 at 1.762µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human non-hodgkin's lymphoma. The section was incubated with ab271287 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] (ab271287)](https://www.abcam.co.jp/ps/products/271/ab271287/Images/ab271287-2-anticd85a-antibody-fras92b-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LILRB3 antibody [FRAS92B] (ab271287)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling LILRB3 with ab271287 at 1.762µg/ml followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Positive staining on human tonsil. The section was incubated with ab271287 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunocytochemistry - Anti-LILRB3 antibody [FRAS92B] (ab271287)](https://www.abcam.co.jp/ps/products/271/ab271287/Images/ab271287-3-anticd85a-antibody-fras92b-immunocytochemistry-293t.jpg)
Immunocytochemistry - Anti-LILRB3 antibody [FRAS92B] (ab271287)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T cells labelling LILRB3 with ab271287 at 3.524µg/ml , followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
Confocal image showing membranous and cytoplasmic staining on 293T cells transfected with LILRB3-myc plasmid.
Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain Myc at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 dilution. -
![Flow Cytometry - Anti-LILRB3 antibody [FRAS92B] (ab271287)](https://www.abcam.co.jp/ps/products/271/ab271287/Images/ab271287-4-anticd85a-antibody-fras92b-flow-cytometry-293t.jpg)
Flow Cytometry - Anti-LILRB3 antibody [FRAS92B] (ab271287)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with myc tagged LILRB3 construct cells labelling LILRB3 with ab271287 at 0.881µg/ml (Right panel) compared with a rat monoclonal IgG isotype control (Left panel). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
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![Anti-LILRB3 antibody [FRAS92B] (ab271287)](https://www.abcam.co.jp/ps/products/271/ab271287/Images/ab271287-5-benefits-of-recombinant-antibodies.png)
Anti-LILRB3 antibody [FRAS92B] (ab271287)
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
参考文献 (0)
ab271287 は論文での使用が確認できていません。
