Anti-LIF 抗体 (ab113262)
Key features and details
- Rabbit polyclonal to LIF
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-LIF antibody
LIF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to LIF -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat -
免疫原
A 16 amino acid synthetic peptide corresponding to internal sequence amino acids of Human LIF (NP_002300).
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ポジティブ・コントロール
- WB: NIH/3T3 and EL4 cell lysates. Mouse colon and rat lung tissue lysates; ICC/IF: NIH/3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab113262 is purified by affinity chromatography via a peptide column. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113262の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
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ICC/IF |
Use a concentration of 20 µg/ml.
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特記事項 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa). |
ICC/IF
Use a concentration of 20 µg/ml. |
ターゲット情報
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機能
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes. -
配列類似性
Belongs to the LIF/OSM family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 16878 Mouse
- Entrez Gene: 60584 Rat
- SwissProt: P09056 Mouse
- SwissProt: P17777 Rat
- Unigene: 4964 Mouse
- Unigene: 44379 Rat
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別名
- CDF antibody
- Cholinergic Differentiation Factor antibody
- D factor antibody
see all
画像
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All lanes : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : EL4 (mouse lymphoma cell line) cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Diluting buffer: 5% NFDM/TBST.
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Immunofluorescence analysis of 3T3 cells, staining LIF with ab113262 at 20 µg/ml.
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Lane 1 : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 2 : Anti-LIF antibody (ab113262) at 2 µg/ml
All lanes : 3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 22 kDa -
Lane 1 : Anti-LIF antibody (ab113262) at 1 µg/ml
Lane 2 : Anti-LIF antibody (ab113262) at 2 µg/ml
All lanes : Rat lung tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG (HRP) at 1/10000 dilution
Predicted band size: 22 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?Diluting buffer: 5% NFDM/TBST.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (18)
ab113262 は 18 報の論文で使用されています。
- Miyazaki Y et al. Potential Metabolite Markers for Pancreatic Cancer Identified by Metabolomic Analysis of Induced Cancer-Associated Fibroblasts. Cancers (Basel) 14:N/A (2022). PubMed: 35326527
- Chen Q et al. Leukemia inhibitory factor regulates Schwann cell proliferation and migration and affects peripheral nerve regeneration. Cell Death Dis 12:417 (2021). PubMed: 33888681
- Wang G et al. Effects of bone marrow mesenchymal stem cells on repair and receptivity of damaged endometrium in rats. J Obstet Gynaecol Res 47:3223-3231 (2021). PubMed: 34184363
- Xie K et al. Downregulation of miR-29c promotes muscle wasting by modulating the activity of leukemia inhibitory factor in lung cancer cachexia. Cancer Cell Int 21:627 (2021). PubMed: 34838029
- Kim SE et al. Decursinol from Angelica gigas Nakai enhances endometrial receptivity during implantation. BMC Complement Med Ther 20:36 (2020). PubMed: 32024510