Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. (ab120501)
Key features and details
- Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export.
- CAS Number: 87081-35-4
- Supplied in ethanol (25 μg/ml)
- Form / State: Liquid
- Source: Steptomyces sp.
製品の概要
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製品名
Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. -
製品の詳細
Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export. -
生理活性の詳細
Potent inhibitor of CRM-1-mediated (exportin-1) nuclear export. Displays antibiotic, antifungal, and antitumor properties and blocks the cell cycle at G1 and G2 phases. Potent inhibitor of nucleo-cytoplasmic translocation of Rev. Also shown to suppress HIV-1 replication in human monocytes (IC50 = 600 pM).
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特記事項
Supplied in ethanol (25 µg/ml).
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CAS 番号
87081-35-4 -
構造式
製品の特性
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体系名
(2E,5S,6R,7S,9R,10E,12E,15R,16Z,18E)-19-[(2S,3S)-3,6-Dihydro-3-methyl-6-oxo-2H-pyran-2-yl]-17-ethyl-6-hydroxy-3,5,7,9,11,15-hexamethyl-8-oxo-2,10,12,16,18-nonadecapentaenoic acid -
分子量
540.74 -
分子式
C33H48O6 -
PubChem 登録番号
6917907 -
保存方法
Store at -20°C (desiccating conditions). -
溶解性
Supplied in ethanol (25 μg/ml) -
使用に関する注意
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
O=C(O)C=C(/C)C[C@H](C)[C@@H](O)[C@H](C)C(=O)[C@H](C)C=C(/C)C=CC[C@@H](C)/C=C(C=C[C@@H]1OC(=O)C=C[C@@H]1C)CC -
由来
Steptomyces sp.
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120501の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120501, Leptomycin B (ethanol solution), Antibiotic, antifungal. Inhibitor of CRM-1 mediated nuclear export.
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ab38335 staining p75 in HeLa cells treated with Leptomycin B (ethanol solution) (ab120501), by ICC/IF. Increase in p75 expression in the nucleous and cytoplasm correlates with increased concentration of Leptomycin B (ethanol solution), as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab120501 (Leptomycin B (ethanol solution)) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab38335 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (2)
ab120501 は 2 報の論文で使用されています。
- John SP et al. IFIT1 Exerts Opposing Regulatory Effects on the Inflammatory and Interferon Gene Programs in LPS-Activated Human Macrophages. Cell Rep 25:95-106.e6 (2018). PubMed: 30282041
- Özdas S & Özdas T Crm1 knockdown by specific small interfering RNA reduces cell proliferation and induces apoptosis in head and neck cancer cell lines. Turk J Biol 42:132-143 (2018). PubMed: 30814875